Studies on te regulatory mechanisms of gene expression in early embryos

1)卵母细胞成熟过程中的基因表达A）成熟培养基影响体外培养的猪卵母细胞的H1 K活性波动模式和生物学特性。B）卵母细胞-卵丘细胞复合体影响在输卵管中传代过程中输卵管壁的结构。2）受精过程中的基因表达A）小鼠顶体酶基因中靶向突变的纯合子雄性小鼠仍然具有生育能力。B）当精子在补充BSA的培养基中预孵育时，用从卵丘-卵母细胞复合物中涡旋出来的可溶性因子补充受精培养基提高了受精率。C）来自c-mos缺陷小鼠的卵母细胞成熟至第二减数分裂中期，但在没有受精的情况下被激活。3）早期胚胎发生期间的基因表达A）类固醇，孕酮，B）ET-1^<-/->纯合子小鼠在出生时死于呼吸衰竭，并且具有咽弓衍生的颅面组织和器官的形态学异常。

1) Gene expression during oocyte maturationA) Maturation medium influences the fluctuation pattern of H1K activity and the biological characteristics of porcine oocytes cultured in vitro.B) Oocyte-cumulus cell complexes influence the structure of the oviductal wall during the passage in the oviduct.2) Gene expression during fertilizationA) Male mice homozygous for a targeted mutation in the mouse acrosin gene were still fertile.B) Supplementing the fertilization medium with soluble factor (s) vortexed out of cumulus-oocyte complexes improved the rate of fertilization when spermatozoa were preincubated in BSA-supplemented medium.C) Oocytes from c-mos-deficient mice matured to the second meiotic metaphase, but were activated without fertilization.3) Gene expression during early embryogenesisA) A steroid, progesterone, was potent to induce parthenogenetic development of unfertilized eggs.B) ET-1^<-/-> homozygous mice die of respiratory failure at birth and have morphological abnormalities of the pharyngeal-arch-derived craniofacial tissues and organs.