Fibroblast growth factor in saliva and oral lesions

我们进行了以下实验，并测定了不同病理生理条件下免疫定位的酸性/碱性成纤维细胞生长因子(aFGF/bFGF)和唾液中的成纤维细胞生长因子水平。用两种单抗建立的双抗体夹心ELISA法，可实现对0.1 ng/ml碱性成纤维细胞生长因子的检测灵敏度。唾液腺组织中碱性成纤维细胞生长因子的浓度为1.1 ng/ml，是正常人血清浓度的5~6倍。为了确定碱性成纤维细胞生长因子在牙龈创面愈合中的作用，我们用免疫过氧化物酶和免疫荧光法以及福尔马林固定的石蜡切片，检测了含有不同阶段肉芽组织的牙周炎手术材料。在肉芽组织中期，碱性成纤维细胞生长因子和硫酸乙酰肝素蛋白多糖共同免疫定位于新合成的基质中。巨噬细胞、肥大细胞和血管内皮细胞也有表达。BFGF阳性巨噬细胞在中期以数量增多为主。这些结果表明，当肉芽组织在新生血管中活跃时，bFGF会从一些巨噬细胞和肥大细胞释放到细胞外环境中，但对纤维化尚不活跃。然而，在它真正应用于唾液测试之前，我们还需要更多的基础准备。

We have carried out the following experiments and determined the immunolocalization acidic/basic fibroblast growth factors (aFGF/bFGF) in various pathophysiologic conditions and the salivary FGF levels.Salivary FGF level ; Using ELISA method, we determined bFGF in the human saliva and the bovine submandibular gland tissue. We could realize the detection sensitivity of 0.1 ng/ml of bFGF by the sandwith ELISA using two kinds of monoclonal antibodies. bFGF was enriched in salivary gland tissues at concentration of 1.1 ng/ml, that was 5 to 6 times of the normal human serum level. However, bFGF was not detected in the saliva with this system.In order to determine the role of bFGF in gingival wound healing, we examined surgical materials of epulides containing various stages of granulation tissue, using the immunoperoxidase and immunofluorescence and formalin fixed paraffin sections. bFGF and heparan sulfate proteoglycan were co-immunolocalized in newly synthesized stroma in the middle organizing stage of granulation tissue. bFGF was also immunolocalized in macrophages, mast cells and vascular endothelial cells. bFGF-positive macrophages mostly increased in number at the middle stage. These findings suggest that bFGF is released from some of macrophages and mast cells into the extracellular milieu when granulation tissue is active in neovascularization but not yet for fibrosis.Application of FGF determination to the clinical examination has not yet developed. However, we need more fundamental preparation before it is really applied to the saliva test.