AUTOMATED MULTI-CHANNEL LUMINOMETER FOR BIOLUMINESCENCE,DESIG AND ITS APPLICATION FOR CIRCADIAN RHYTHMICITY

利用16通道光罩、光电倍增管、袖珍计算机和Macintosh计算机，研制了自动化多通道光度计，并编制了仪器控制和数据分析程序。该仪器允许遵循两种不同生物体的昼夜节律。多edra膝沟藻（Gonyaulax polyedra）在生物发光、细胞分裂和光合作用等方面具有明显的昼夜节律性。在恒定光照条件下，它表现出节律性，周期约为23小时，但我们不能检测到肌酸的缩短周期的作用，由我们的光度计。由于影响节律性的因素很多，我们无法在各种条件下（光照强度，监测时间，温度等）重现报道的效果。考察基因操纵的蓝藻（引入细菌荧光素酶）显示生物发光。光照强度低于甲藻，但具有明显的昼夜节律性，并具有温度补偿等重要特征。

Automated mutli-channel luminometer was made by using 16 channel housing, photomultiplier, pocket computer, and Macintosh computer and developted the programs for controlling the machine and analyzing the obtained data. This instrument allowed to follow circadian rhythmicity of two different organisms. Bioluminescent dinoflagellate Gonyaulax polyedra is well know to show circadian rhythmicities in several dinstinct biological events including bioluminescence, cell devision and photosynthetic activity. Under constant light conditions, it showed rhythmicity with a period of about 23 h, however, we could not detect the period-shortening effect of creatine by our luminometor. Since there are many factors influence on the rhythmicity, we could not reproduce the reported effects under various conditions (light intensity, monitoring time, temperature etc.) examined. Gene manipulated cyanobacteria (introducing bacteria luciferase) showed bioluminescence. The light intensity is lower than dinoflagellate but exactly showed circadian rhythmicity with several important characters such as temperature compensation.