Development and physiological role of gammadelta T cells.

虽然携带γ - δ t细胞受体的t细胞长期以来被认为存在于许多器官的上皮内层，但它们的发育、特异性和功能仍然是未知的。在这项研究中，我们分析了定位于小鼠肠上皮的一个主要γ δ t细胞亚群的生理意义。成年小鼠肠上皮内t细胞（IEL）池的大小与脾脏中的t细胞池相当。它由α t细胞（40-70%）和γ t细胞（30-60%）组成。我们的结果表明；1)无菌小鼠的微生物剥夺优先影响α - iel种群减少并变为非细胞溶解性，但对γ - iel的池大小和特征影响不大。2)在aly突变小鼠中，IEL的定植发生在完全缺乏Peyer^<， bbbbs斑块和固有层中产生iga的B细胞的情况下。3) γ - δ - iel缺失与肠上皮细胞周转减少和MHCⅱ类分子表达下调有关。这些结果表明，α -和γ - iel独立于肠上皮中的Peyer^<， bbbbs斑块发育，并且在肠粘膜中的生理功能不同。

Although T-cells bearing gammadelta T-cell receptors have long been known to be present in the epithelial lining of many organs, their development, specificity and function remain elusive. In this study, we analyzed the physiological significance of one major gammadelta T-cell subset which is localized in the mouse intestinal epithelium. The pool of intestinal intraepithelial T-cells (IEL) of adult mice is comparable in size to the T-cell pool in the spleen. It consists of both alphabeta T-cells (40-70%) and gammadelta T-cells (30-60%).Our result show ;1)Microbial deprivation in germ-free mice preferentially influences the alphabeta-IEL population to decrease and become noncytolytic but has little effect on the pool size or characteristics of gammadelta-IEL.2)Colonization of IEL in the aly mutant mice takes place in the complate absence of Peyer^<, >s patches and IgA-producing B cells in lamina propria.3)The absence of gammadelta-IEL is associated with a reduction in intestinal epithelial cell turnover and a downregulation of the expression of MHC class II molecules.These results indicate that alphabeta- and gammadelta-IEL develop independently of Peyer^<, >s patches in the intestinal epithelium and differ from each other in their physiological function at the gut mucosa.