Study on the way of protection and prevention of HFRS in laboratory animal experiments.

我们发现裸鼠对汉坦病毒感染非常敏感。为了研究汉坦病毒感染的机制，采用裸鼠作为检测感染的指标。首先，我们通过注射不同数量的活性病毒产生了具有不同抗体滴度的大鼠。总体而言，抗体滴度较低的大鼠对指标裸鼠的感染性较抗体滴度较高的大鼠低。但实验感染汉坦病毒B-1株的大鼠，除抗体效价低于x128的大鼠外，几乎所有大鼠均对裸鼠具有感染性。采用RT-PCR检测病毒基因组RNA。本实验条件下的检出限为0.1 pfu。基因组RNA在病毒感染的早期和再激活阶段被检测到。然而，即使抗体滴度很高，在感染后期也无法检测到。在实验感染的大鼠中，免疫印迹检测均呈阳性，均可感染裸鼠。

We found that nude rat was very sensitive in the hantavirus infection. In order to study the mechanism of hantavirus infection, nude rats were used as an indicator of the detection of infection.First of all, we have produced rats having different antibody titers by injecting different number of active viruses. In general, rats having lower antibody titers showed lower infectivity to indicator nude rats in comparison with the one having higher titers. However, almost all of the rats experimentally infected with hantavirus B-1 strain showed infectivity to nude rats except the rats having the antibody titer less than x128 for all the time observed. To check the virus genome RNA,RT-PCR was performed. The detection limit was 0.1 pfu in our experimental condition. Genome RNA was detected in the early phase of viral infection and also in reactivation phase. However, it was not detected at the later stage of infection even if the antibody titer was high. In this experimentally infected rats, all showed positive in western blotting were infective to nude rats.