Prevention of cattle-botulism in Australia

C型和D型肉毒杆菌菌株是从澳大利亚一个养牛场（牧场）的土壤中分离出来的。从一个C型培养物中纯化出C型毒素，用单克隆抗体分析其抗原性。根据抗原性和毒素中和试验结果，认为毒素的c端可以作为有效的疫苗。合成了在不同类型肉毒杆菌毒素轻链组分中保守的肽（15个氨基酸残基），并在大鼠体内制备了针对其的单克隆抗体。虽然获得了两种与任何类型毒素反应的单克隆抗体，但它们都不能中和毒素，说明该肽不能用作疫苗，但由此获得的单克隆抗体可用于任何类型毒素引起的肉毒中毒的诊断。

The type C and D botulinum strains were isolated from the ground of a cattle farm (a pasture) in Australia. The type C toxin was purified from one type C culture, and its antigenicity was analyzed by monoclonal antibodies. Based on the results of antigenicity and toxin-neutralization tests, it was concluded that the C-terminal of the toxin can be used as effective vaccine.The peptide (15 amino acid residues) which is conserved in the light chain components of any different types of botulinum toxins was synthesized, and the monoclonal antibodies against it were prepared in rats. Though two monoclonal antibodies reacting with any types of toxins were obtained, they did not neutralize the toxigenicity, indicating that this peptide can not be used as vaccine, but the monoclonal antibodies thus obtained can be used for diagnosis of botulism caused by any types of toxins.