Studies on Dynamic and Functional Aspects of Plant Ecochemicals

该研究项目侧重于植物次生代谢物的动态和功能方面，涵盖了非常广泛的研究领域。据报道，该项目三年来取得的成果包括61篇研究论文，其中包括3篇在刊论文和2篇已提交的论文，以及2本书和83篇口头报告。本文的研究成果概括如下：(1)建立新的核磁共振技术，应用于生物活性天然产物的结构鉴定，生物活性胺和钙的原位分析方法的发展(11篇论文)；(2)花卉和马铃薯花青素以及高等植物中新的多酚和萜类化合物的结构解析和定量(13篇论文)；(3)具有α-葡萄糖苷酶抑制和抗氧化活性的植物次生代谢物及其结构、构效关系和作用方式(5篇论文)。(4)植物防御剂：蔷薇科植物的化感作用、杀虫拒食原理和抗菌物质(18篇论文，(6)植物与病原微生物、共生微生物和附生微生物相互作用的化学研究(3篇)。通过大量的研究，我们现在可以更好地了解植物生态化学物质在以自养植物和异养生物为基础的生态系统中发挥多种作用的意义。我们相信，进一步研究植物生态化学物质的必要性越来越大，以找到低投入可持续农业的方法，以及找到我们新的生活方式，与地球上的其他生物永远共存。

The research project focusing on dynamic and functional aspects of plant secondary metabolites covers very wide research areas. The results achieved in the project for three years have been reported as 61 reseach papers including 3 papers in press and 2 papers submitted, and 2 books, in addition to 83 oral presentations. Our reseach results are summarized as follows :(1) Establishment of new NMR techniques and application to structure elucidation of biologically active natural products, and development of in situ analytic methods of bioactive amines and calcium (11 papers).(2) Structure elucidation and quantification of anthocyanins in flowers and potatoes, and new polyphenols and terpenoids in higher plants (13 papers).(3) Plant secondary metabolites possessing alpha-glucosidase inhivitory and antioxidative activities, and their structure, structure-activity relationships and mode of action (5 papers).(4) Plant defense agents : allelopathic, insecticidal and antifeeding principles, and antimicrobial substances in the family Rosaceae (18 papers, 2 books).(5) Studies on biosynthesis, metabolism and functions of flavonoids as general defense substances in plants (6 papers).(6) Chemical studies on interactions between plant and pathogenic, symbiotic and epiphytic microorganisms (3 papers).Through such a lots of researches, we can now understand a little well about the significancy of plant ecochemicals taking many kinds of roles among living substances in the ecosystem based on autotrophic plants and heterotrophic organisms. It is our confidence that the necessity of further studies on plant ecochemicals is increasing more and more to find the way to low-input sustainable agriculture as well to find our new life style to coexist forever with other organisms on the earth.