Structures, Permeabilties, and Cell Attachment and Growth of Tough and High Water Content PVA/(Water Soluble Polymer) Blend Hydrogels.

将聚乙烯醇（PVA）与另一种水溶性高分子聚合物共混，制备了具有坚韧和橡胶状物理交联的水凝胶。e.将阴离子型聚L-谷氨酸钠（PSLG）和聚苯乙烯磺酸钠（NaPSS）、阳离子型壳聚糖、中性聚乙二醇（PEG）和聚丙烯酰胺（PAAm）在高压釜中进行共混。e.自由水的冰、未冻结的水和无序的冰晶。在未冻水凝胶中，冰的无序结晶以水的中间状态存在，水分子间氢键被削弱，分子流动性较低，PVA/NaPSS和PVA/Chitosan水凝胶的透氧系数不依赖于水含量，并且随着半径的增加而增加，并且/或者简单的非常狭窄的可渗透通道，其中水分子具有减弱的分子间氢键力。而PVA/PEG和PVA/PAAm共混水凝胶的渗透系数取决于水凝胶的孔结构，壳聚糖含量低于10wt%的PVA/NaPSS、PVA/PEG、PVA/PAAm和PVA/Chitosan水凝胶几乎没有细胞附着。当壳聚糖含量高于20wt%时，细胞附着和生长随着壳聚糖含量的增加而增加，并且具有40wt%壳聚糖含量的水凝胶超过广泛用作哺乳动物细胞培养基质的胶原。

Tough and rubber-like physically cross-linked hydrogels were prepared by blending of concentrated aqueous solutions of poly (vinyl alcohol) (PVA) and another water soluble polymer i. e. anionic poly (sodium L-glutamate) (PSLG) and poly (stirene sulfonic acid) sodium salt (NaPSS), cationic chitosan, and neutral poly (ethylene glycol) (PEG) and poly (acryl amide) (PAAm), in an autoclave.There are three types of water in every frozen blend hydrogel, i. e. ice of free water, unfrozen water, and disordered crystal of ice. In the unfrozen hydrogels, the disordered crystal of ice exists as an intermediate state of water which is weakened intermolecular hydrogen bonds and has low molecular mobility.The oxygen permeability coefficients of both the PVA/NaPSS and PVA/Chitosan hydrogels did not depend on the water content, and increase with increasing radius and/or simplicity of very much narrow permeable channels in which the water molecules has the weakened intermolecular hydrogen bonding force. While for the PVA/PEG and PVA/PAAm blend hydrogels the permeability coefficients depended on the porous structure in the hydrogels.Every PVA/NaPSS,PVA/PEG,and PVA/PAAm hydrogels and PVA/Chitosan hydrogels containing below 10wt% chitosan content were observed hardly any attached cells. While above 20wt% chitosan content the cell attachment and growth increases with increasing chitosan content and the hydrogel with 40wt% chitosan content exceed collagen which is widely used as a mammalian cell culture substrate.