Application to Material for Cell Growth of Protain Hydorgel

Protain Hydorgel在细胞生长材料中的应用

基本信息

批准号：
14580814
负责人：
NAGURA Masanobu
金额：
$ 2.3万
依托单位：
Shinshu University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2004
项目状态：
已结题

项目摘要

Biomaterials are usually used in solid state. Sericin obtained by using a usual degumming method is low molecular weight, and its film is very brittle and weak mechanical properties. In this study, we focused to obtain tough membrane and to study the relationship between cell growth and its surface properties.At first, we are prepared the blend of sericin obtained by usual degumming of cocoon with poly(vinyl alcohol) that is low molecular weight and low saponification degree and then the membrane was injected. This membrane is good mechanical properties to use biomaterials because of molecular interaction between amorphous chain of poly(vinyl alcohol) and sericin.Secondly, we prepared two kinds of high molecular weight sericin by using mild extraction condition (Sericin-B), namely boiling only in water, and by using a new sericin rich sericin cocoon (Sericin-S). The hydrogel Sericin-B membrane showed better mechanical properties than the usual degummed sericin membrane, but could not handle as hydrogel membrane. While Sencin-S hydrogel membrane was rubbery. The low molecular weight fracton was also contained in the hydorgel membrane, therefore immersing in pure water at body temperature eluted this fraction. After immersing for 5 days the elution is finished and the membrane was weakened on the mechanical properties although it could handle in the, hydrogel. Cell growth was measured, by using mouse 929-fibroblast cell. Sericin-S hydrogel membrane was better cell growth than Sericin-B hydrogel membrane. The contact angles of the water on the Sericin-B and Sericin-S hydrogel membranes are 37 and 71 degrees, respectively. These values mean that Sericin-B is hydrophilic or anionic, while Sericin-S is lower anionic property than Sericin-B. The values of ζ-potential for these membranes showed minus, namely anionic. From the contact angle data, the good cell growth of Sericin-S membrane caused by the convenient contact angle rather than anionic property.

生物材料通常以固态使用。使用通常的脱胶方法获得的丝网膜素是低分子量，其膜非常脆，机械性能。在这项研究中，我们专注于获得坚硬的膜并研究细胞生长及其表面特性之间的关系。首先，我们准备了通过茧与低分子量和低皂化度的聚（乙烯基醇）进行通常的茧中获得的丝氨酸的混合物，然后注入了膜。该膜是使用生物材料的良好机械性能，因为多（乙烯基醇）和丝氨酸之间的无形链之间的分子相互作用。第二，我们通过使用温和的萃取条件（Sericin-b）来制备两种高分子体重塞拉西蛋白（Sericin-B），即仅在水中沸腾，即使用新的Sericin sericinicinicinicinicinicincoon（）。机械性能比通常的脱胶丝质膜，但不能像水凝胶膜一样处理。而Sencin-S水凝胶膜是橡胶。低分子量的fracton也包含在水凝胶膜中，因此在体温下浸没在纯净水中，从而洗脱了这一部分。浸入5天后，洗脱了，尽管可以在水凝胶中处理机械性能，但在机械性能上薄弱。通过使用小鼠929纤维细胞细胞测量细胞生长。 Sericin-S水凝胶膜比Sericin-B水凝胶膜更好的细胞生长。塞他蛋白-B和塞他蛋白-S水凝胶膜上水的接触角分别为37度和71度。这些值意味着Sericin-B是亲水性或阴离子的，而Sericin-S的阴离子特性低于Sericin-B。这些膜的ζ电位值显示为负，即阴离子。从接触角数据中，塞他蛋白-S膜的良好细胞生长是由方便的接触角而不是阴离子特性引起的。