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Application to Material for Cell Growth of Protain Hydorgel

Protain Hydorgel在细胞生长材料中的应用

基本信息

批准号：
14580814
负责人：
NAGURA Masanobu
金额：
$ 2.3万
依托单位：
Shinshu University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2004
项目状态：
已结题

项目摘要

Biomaterials are usually used in solid state. Sericin obtained by using a usual degumming method is low molecular weight, and its film is very brittle and weak mechanical properties. In this study, we focused to obtain tough membrane and to study the relationship between cell growth and its surface properties.At first, we are prepared the blend of sericin obtained by usual degumming of cocoon with poly(vinyl alcohol) that is low molecular weight and low saponification degree and then the membrane was injected. This membrane is good mechanical properties to use biomaterials because of molecular interaction between amorphous chain of poly(vinyl alcohol) and sericin.Secondly, we prepared two kinds of high molecular weight sericin by using mild extraction condition (Sericin-B), namely boiling only in water, and by using a new sericin rich sericin cocoon (Sericin-S). The hydrogel Sericin-B membrane showed better mechanical properties than the usual degummed sericin membrane, but could not handle as hydrogel membrane. While Sencin-S hydrogel membrane was rubbery. The low molecular weight fracton was also contained in the hydorgel membrane, therefore immersing in pure water at body temperature eluted this fraction. After immersing for 5 days the elution is finished and the membrane was weakened on the mechanical properties although it could handle in the, hydrogel. Cell growth was measured, by using mouse 929-fibroblast cell. Sericin-S hydrogel membrane was better cell growth than Sericin-B hydrogel membrane. The contact angles of the water on the Sericin-B and Sericin-S hydrogel membranes are 37 and 71 degrees, respectively. These values mean that Sericin-B is hydrophilic or anionic, while Sericin-S is lower anionic property than Sericin-B. The values of ζ-potential for these membranes showed minus, namely anionic. From the contact angle data, the good cell growth of Sericin-S membrane caused by the convenient contact angle rather than anionic property.

生物材料通常以固态使用。采用常规脱胶方法得到的丝胶分子量低，胶膜易碎，机械性能差。为了获得坚韧膜，并研究细胞生长与膜表面性质的关系，本研究首先将蚕茧常规脱胶所得的丝胶与低分子量、低皂化度的聚乙烯醇（PVA）共混，然后将共混物注入膜中。由于聚乙烯醇的无定形链与丝胶分子间的相互作用，该膜具有良好的力学性能，可用于生物材料。其次，我们采用温和的提取条件（即水煮法）（Sericin-B）和一种新的富含丝胶的蚕茧（Sericin-S）制备了两种高分子量的丝胶。与常规脱胶膜相比，水凝胶膜具有更好的力学性能，但不能作为水凝胶膜处理。而Sencin-S水凝胶膜是橡胶状的。低分子量组分也包含在水凝胶膜中，因此在体温下浸入纯水中洗脱该组分。浸泡5天后，洗脱完成，膜的机械性能减弱，尽管它可以在水凝胶中处理。通过使用小鼠929-成纤维细胞测量细胞生长。Sericin-S水凝胶膜比Sericin-B水凝胶膜具有更好的细胞生长。水在丝氨酸-B和丝氨酸-S水凝胶膜上的接触角分别为37度和71度。这些值意味着丝氨酸-B是亲水性或阴离子性的，而丝氨酸-S的阴离子性低于丝氨酸-B。这些膜的膜电位均为负，即阴离子型。从接触角数据来看，Sericin-S膜良好的细胞生长是由方便的接触角而不是阴离子性质引起的。