Control of Cell Functions by Functioralized Liposomes

首次研究了表达于小鼠畸胎瘤F9上的细胞间黏附分子E-钙粘附素从细胞到脂质体的转移。改变了脂质体的脂组成和与细胞孵育的条件，优化了膜蛋白的转移。为了证实E-钙粘蛋白从脂质体到另一个细胞的再转移，将含有E-钙粘素的膜蛋白通过膜间蛋白转移与脂质体共同孵育在Jurkat细胞和B16黑色素瘤细胞上。这些细胞最初不与F9细胞黏附，但与脂质体共孵育后获得了与F9细胞的黏附活性。这表明E-钙粘蛋白可以从脂质体转移到其他细胞膜上，保持其黏附活性，这意味着E-钙粘蛋白的引入成功地改变了细胞的功能。将两种培养的哺乳动物细胞培养在2.0ml的Vol试管中，该试管配备了专门设计的氧电极来测量培养液中的氧浓度([02])。这种新方法可以1)即时测定活细胞的呼吸活动，2)同时监测细胞对细胞外刺激的反应。

Transfer of E-cadherin, which is a cell-cell adhesion molecule and expressed on mouse teratocarcinoma F9, from the cell to liposome was first investigated. The lipid composition of liposome and the condition of the incubation with cell were altered, and the transfer of membrane proteins was optimized. For the demonstration of the retransfer of E-cadherin from liposome to another cell, then cells such as Jurkat cell or B 16 melanoma were coincubated with the liposome on which membrane proteins containing E-cadherin was reconstituted by intermembrane protein transfer. Originally these cells do not adhere to F9 cell, however they acquired the adhesion activity to F9 cell after coincubation with the liposome. It was suggested that E-cadherin was transferred from the liposome to other cell membranes keeping its adhesion activity, and meant that the function of cells were successfully modified by the introduction of E-cadherin.A new method to determine oxygen uptake rate of living cells is described. Two kinds of cultured mammalian cells were incubated in a 2.0 ml vol cuvette, which was equipped with an oxygen electrode as specially designed to measure the oxygen concentration ([02]) in the culture medium. This new method allowed 1) instant determination of the respiratory activity of living cells, and 2) simultaneous monitoring of responses of cell against extracellular stimuli.