Elucidation of Ion Transport Process and Oscillation Processes Through a Bilayr Lipid Membrane and Its Application to Analytical Chemistry.

采用插入辅助参比电极的液膜玻璃池，阐明了离子通过膜的伏安图与两个水/膜界面处的伏安图之间的相关性。膜转运被发现是由两个水/膜界面的互补离子转移控制。上述方法和概念被证明是可用的，以分析膜的特征反应，如两个不同的电荷转移反应之间的相互依赖性进行在各自的界面或膜电位和膜电流的振荡。当BLM含有足够的离子时，发现通过BLM的离子传输类似于通过液膜的离子传输，尽管BLM比液膜薄得多。应该强调的是， ...更多信息 当Na ^+从一种含0.1M NaCl的水溶液（W1）通过含稀Na^+的液膜（LM）转移到另一种无Na^+的水溶液（W2）时，观察到膜电流或膜电位的新振荡分别由施加的膜电位或膜电流强制。在W1中加入乙酰胆碱可引起电流振荡，而在W1中加入四烷基铵离子或谷氨酸离子等疏水性离子则可抑制电流振荡。这些振荡与具有所谓的“钠通道”的生物膜上的那些振荡在诱导和抑制方面类似，尽管LM系统不包含任何通道蛋白。通过对离子在膜中及水/膜界面上迁移的伏安图分析，并考虑到Na^+在LM/W2界面上的吸附，阐明了振荡、诱导和抑制的机理，并对水/有机溶液界面上的电子转移反应进行了研究，离子和电子在液膜中的耦合传输以及离子对萃取平衡的定量解释。少

The correlation between the voltammogram for ion transfer through a membrane and those at two aqueous/membrane interfaces was elucidated by employing a glass cell with a liquid membrane into which an auxiliary reference electrode was inserted. The membrane transport was found to be controlled by the complementary ion transfers at two aqueous/membrane interfaces. The above methodology and concept were demonstrated to be available to analyze the characteristic reactions of the membrane, like the interdependency between two different charge transfer reactions proceeding at the respective interfaces or the oscillation of membrane potential and membrane current.The ion transport through a bilayr lipid membrane (BLM) was investigated voltammetrically. The ion transport through a BLM was found to be analogous to that through a liquid membrane when the BLM contains sufficient ions, although the BLM is very much thinner than the liquid membrane. It should be stressed that the spontaneous conden … More sation of objective ions from aqueous solution (s) into the BLM by the partition with counter ions is essential for the ion transport through BLMs.Novel oscillations of membrane current or membrane potential were observed when the transfer of Na^+ from one aqueous solution (W1) containing 0.1 M NaCl to another (W2) in the absence of Na^+ through a liquid membrane (LM) containing dilute Na^+ was forced by an applied membrane potential or membrane current, respectively. The current oscillation was induced by the addition of acetylcholine in W1, and inhibited by the addition of such hydrophobic ions as tetraalkylammonium ions or glutamate ion in W1. These oscillations were similar to those at biomembranes with the so-called "sodium channel" as regards both induction and inhibition, though the LM system did not contain any channel proteins. The mechanisms of the oscillations, the induction and the inhibitions were elucidated by referring to voltammograms for the ion transfer through a membrane as well as those at aqueous/membrane interfaces, and by taking into account the adsorption of Na^+ at the LM/W2 interface.In addition, investigations have been carried out for the electron transfer reactions at the aqueous/organic solutions interface, the coupling between the ion and electron transports through a liquid membrane and the quantitative interpretation on the ion-pair extraction equilibrium. Less