Physiological functions of crops transformed with Agrobacterium spp. and its agricultural use

1.日本农杆菌属60个野生菌株。从多花蔷薇（Rosa multiflora Thunb.）富山地区的蔷薇属植物和栽培品种。将它们编号并作为TPU（富山县立大学）的菌株接受。其中TPU-50、TPU-54、TPU-55和TPU-57菌株经细菌学鉴定为根癌农杆菌生物变种2（Agrobacterium tumefacience biovar 2），其余菌株与农杆菌属（Agrobacterium spp.）Southern杂交分析结果表明，该菌株可分为5个独特的T-DNA类型，与传统的农杆菌NIAES 1724和A13不同。TPU-菌株转化组织中冠瘿碱的类型为胭脂碱型、农根碱型和米希莫平型。几乎所有的TPU菌株都有rol B和rol C基因.在转化组织中细胞分裂素和脱落酸的含量方面，冠瘿和下胚轴外植体组织中玉米素核苷和顺式脱落酸含量相对较高.当TPU-57或TPU-58菌株的细菌细胞接种于从种子繁殖甘薯获得的下胚轴切片的顶部时，在接种表面上观察到肿瘤或愈伤组织形成。继代培养50天后，在含有万古霉素和羧苄青霉素的MS培养基上，获得了转化植株，在此基础上，我们希望进一步研究TPU菌株转化作物植株中内源细胞分裂素和阿坝的变化。

1. Sixty Japanese wild-strains of Agrobacterium spp. were isolated from crown galls of Rosa multiflora thunb. and cultivars of Rosa in Toyama districts. they were numbered and accepted as the strains of TPU (Toyama Prefectural University).2. Among their TPU strains, TPU-50, -54, -55 and-57 strains were identified as Agrobacterium tumefacience biovar 2 from the date of bacteriological natures, and other strains were different from Agrobacterium spp.3. The results of southern hybridization analysis showed that the TPU-strains isolation here could be classified into five unique T-DNA types distinct from authentic Agrobacterium strains, NIAES 1724 and A13 types. Opine type in tissues transformed with TPU- strains was nopaline, agropine and mikimopine types. Almost all of TPU-strains had rol B and rol C genes.4. As for the contents of cytokinins and abscisic acid in transformed tissues, the crown gall and hypocotyle explant tissues had relatively high amount of zeatin riboside and cis-abscisic acid.5. When the bacterial cells of TPU-57 or TPU-58 strains were inoculated on the top of the hypocotyle sections obtained from seed-propagation Sweet potato, tumor or callus formation were observed on the inocutated surface. After about 50 days of subcul turing, the transformed plants were regenerated from these callus on MS medium containing vancomycin and carbenicillin.On the results obtaineed here, we wish to study in future adout the alterations of endogenous cytokinins and ABA(s) in crop plants transformed by TPU-strains.