Research on Nitrification : Isolation and Suppression of Nitrifing Bacteria

(1)尽管制备具有不同取代基的1,3,5-三嗪比较困难，但2,4-二（三氯甲基1）-6-三氟甲基1-1,3,5-三嗪和2-甲基1-4-三氯甲基1-6-三氟甲基1-1,3,5-三嗪是通过相应的酰亚胺与酸酐的缩合反应合成的。其他许多1,3,5-三嗪类化合物是通过三聚反应和共三聚反应合成的。(2)含氟取代基的1,3,5-三嗪具有较弱的硝化抑制活性，而2-氨基-4-三溴二甲基1-6-三氯二甲基1-1,3,5-三嗪对欧洲亚硝化单胞菌ATCC25978的抑制作用非常强（pI_<50>=7.12）。其对硝化反应的抑制作用是原硝化抑制剂MAST的65倍（pI_<50>=5.31）。(3)制备了一种新的培养N.europaea ATCC25978的碳酸氢铵（ABC）培养基。在这种培养基中，欧洲草的生长速度比在Lewis & Pramer培养基中快3倍。(4)利用ABC培养基从土壤中分离新的硝化细菌的尝试尚未成功。(5)利用N.europaea ATCC25978， N.sp.；TK794, Nitorobacter agile ATCC14123，和N.sp TH21。合成的1,3,5-三嗪类化合物抑制Nitorosomonas属的生长。(6)在以硫酸铵为底物的N.europaea消耗o_2的细胞游离水平测定中，发现合成1,3,5-三嗪的靶酶为氨单加氧酶。

(1)In spite that it is difficult to prepare 1,3,5-triazines having different substituents, 2,4-bis(trichloromethy1)-6-trifluoromethy 1-1,3,5-triazine and 2-methy 1-4-trichloromethy 1-6-trifluoromethy 1-1,3,5-triazine were synthesized by the condensation reaction between corresponding imidoy 1-amidines and acid anhydrides. Other many of 1,3,5-triazines were synthesized using trimerization reaction and co-trimerization reactions.(2)1,3,5-Triazines having fluorine substituents showed weak nitrification inhibitory activity but 2-amino-4-tribromomethy 1-6-trichloromethy 1-1,3,5-triazine very strongly suppressed the growth of Nitrosomonas europaea ATCC25978 (pI_<50>=7.12). It was 65 times stronger than that of authentic nitrification inhibitor, MAST (pI_<50>=5.31).(3)We made a new ammonia bicarbonate (ABC) medium for the culture of N.europaea ATCC25978. In this medium N.europaea grew 3 times faster than in Lewis & Pramer medium.(4)Attempt to isolate new nitrifying bacteria using ABC medium from soil is not sucessful yet.(5)In the bioassay using N.europaea ATCC25978, N.sp.TK794, Nitorobacter agilis ATCC14123, and N.sp TH21., synthesized 1,3,5-triazines inhibited the growth of genus Nitorosomonas.(6)In the cell free level assay of O_2-consumption by N.europaea using ammonium sulfate as substrates, the target enzyme of synthesized 1,3,5-triazines is found to be an ammonia-monooxygenase.