DIRECT IMAGING OF E.coil POLYMERASE AND TRANSCRIPTION COMPLEX WITH PROMOTER REGION OF MUTANT DNA (UV5).

改进的低角度旋转阴影可用于RNA聚合酶的结构分析及其与DNA的结合（转录复合）。聚合酶的阴影图像基本上由两种不同尺寸和一种类似结构的四个亚单位组成。两个大子单元的尺寸结构堆叠在另一个小尺寸结构上，形成了一个大信道，宽度为3纳米。Rod在这个频道上找到了一个结构。大的和小的子单位似乎对b， b^和2个子单位的反应是正确的，从分子重量中的差异中判断出来，这已经证明了生物化学程度如此之远。在与DNA的促进者区域绑定上，DNA链在这个大频道中被发现。Compa\observation of core enzyme without s factor suggested that s factor should be rod like structure placed along this channel together with DNA。Another narrow space was recognizable between b and b^ユ subunits。将mRNA的b个亚单位的关联性输入到帐户中，这个切片可能是新合成mRNA的通道。

Improved low angle rotary shadowing was available for structural analysis of RNA polymerase and its binding to DNA (transcription complex). The shadowed image of the polymerase was basically composed of four subunits in two different sizes and one rod like structure. A dimeric structure of two big subunits was stacked on another small dimeric structure, forming large channel between them, 3nm in width. Rod like structure was found in this channel. Big and small subunits seem to correspond to b, b^ユ and 2 a subunits respectively, judging from differences in their molecular weight that have been revealed biochemically so far. On binding with the promoter region of DNA,DNA strand was found in this large channel. Comparative observation of core enzyme without s factor suggested that s factor should be rod like structure placed along this channel together with DNA.Another narrow space was recognizable between b and b^ユ subunits. Taking the affinity of b subunit to mRNA into account, this slit may be a channel for newly synthesizing mRNA.