The effect of hyaluronic acid on experimental temporomandibular joint osteoarthrosis in the sheep

目的：本研究旨在探讨透明质酸（HA）反复注射对绵羊颞下颌关节（TMJ）骨关节病（OA）模型的影响。分别于术后7、10、14、17、21天在一侧关节内注射HA。对侧关节内注射生理盐水。1月龄时宰杀3只，3月龄时宰杀3只。取出关节并进行肉眼和组织学检查。一个特殊的评分系统应用以下的修改Mankin的评分，以评估组织学changes.Results：对照组表现出严重的骨关节病的变化，在髁状突，偏离形式从正常形态，和显着的骨髓纤维化。HA治疗组与正常髁突形态的偏离较小。1个月时的组织学评分为HA 12.6，对照组24.2（p<.001），3个月时为HA 6.9，对照组18.9（p<.001）。有一个显着的差异，HA治疗和控制TMJ之间的骨关节炎的变化，HA治疗TMJ具有较低的严重changes.Conclusion：反复关节内注射HA到羊TMJ与实验诱导OA最大限度地减少骨关节炎的变化程度时，与对照关节。因此，HA可能在预防TMJ OA进展中发挥作用。

Purpose : The purpose of this study was to test the effect of repeated injections of hyaluronic acid (HA) on the sheep model of osteoarthrotic temporomandibular joint (TMJ) disease.Materials and Methods : Bilateral osteoarthrosis (OA) was induced in the TMJs of six sheep. HA was injected into one joint on 7,10,14,17, and 21 days postoperatively. Normal saline was injected into the contralateral joint. Three sheep were killed at 1 month and 3 at 3 months. The joints were removed and examined macroscopically and histologically. A special scoring system was applied following the modified Mankin's score to evaluate the histologic changes.Results : The control group showed severe osteoarthrotic changes in the condyle, deviation in form from normal morphology, and marked marrow fibrosis. The HA-treated group showed less deviation from normal condylar morphology. The histologic scores at 1 month were HA 12.6, control 24.2 (p<.001), and at 3 months were HA 6.9, control 18.9 (p<.001). There was a significant difference in osteoarthrotic changes between HA-treated and control TMJs, with the HA-treated TMJs having less severe changes.Conclusion : Repeated intraarticular injections of HA into a sheep TMJ with experimentally induced OA minimizes the extent of osteoarthrotic change when compared with the control joint. Thus, HA may have a role in preventing the progression of TMJ OA.