Etiological study of oral disease in HIV and HTLV-I positive patients -characteristics of salivary gland cells-

人类T淋巴营养病毒I型（HTLV-I）参与干燥综合征（SS）的发病机制，这在hiv感染患者中也可见，在本研究中得到澄清。对1992年至1996年间转诊到九州大学牙科医院口腔颌面外科第二科的103例血清HTLV-I阳性SS患者中的11例进行了研究。细胞DNA的聚合酶链反应（PCR）扩增表明，从唇唾液腺（SG）提取的细胞DNA在SG中含有完整的HTLV-I前病毒DNA。在SG切片中检测HTLV-I前病毒DNA的原位PCR杂交显示，HTLV-I前病毒DNA在浸润的T细胞的细胞核中，但在唾液腺上皮细胞和腺泡细胞中均未发现。利用人htlv - 1感染的T细胞系MT2细胞和唾液腺上皮细胞系对htlv - 1前病毒DNA进行定量分析，发现SG中的病毒载量比外周血单个核细胞高约8 ~ 9 * 10^4倍。因此，SG中有htlv -i感染的T细胞积累。HTLV-I可能引起自反应性T细胞增殖，从而诱发SS。

The involvement of Human T lymphotrophic virus type I (HTLV-I) in the pathogenesis of Sjogren' syndrome (SS), which is also seen in HIV-infected patients, was clarified in this study.Eleven of one hundred and three SS patients, who were serologically HTLV-I positive, referred to the Second Department of Oral and Maxillofacial Surgery, Kyushu University Dental Hospital between 1992 and 1996, were studied. A polymerase chain reaction (PCR) amplification of cellular DNA revealed that the cellular DNA extracted from the labial salivary glands (SG) contained full HTLV-I proviral DNA in the SG.In situ PCR hybridization which detect HTLV-I proviral DNA in the section of the SG demonstrated HTLV-I proviral DNA in the nucleus of the infiltrating T cells, but not in either salivary gland epithelial or acinar cells. Quantification of HTLV-I proviral DNA was performed using MT2 cells, a human HTLV-I -infected T cell line and salivary gland epithelial cell lines, and revealed that the viral loads in the SG were approximately 8 times to 9 * 10^4 times higher than those in the peripheral blood mononuclear cells.An accumulation of HTLV-I-infected T cells in the SG is thus suggested. HTLV-I likely cause the self-reactive T cells proliferate and, as a result, thereafter induced SS.