Basic Study of Information Processing in Living Plants

分析完整细胞或组织中的生物活性对于理解植物中的信息处理是必不可少的。这些活体测量技术还没有得到很好的发展，特别是根。我们研究了一种非破坏性的方法，以了解植物中的信息处理，使用中子束，以及正电子发射核素进行PET（正电子发射断层扫描）。中子束技术提供了迄今为止对组织中水分的最高分辨率。这种中子束技术对水有很高的特异性，可以成像种子或埋在土壤中的根以及发育过程中木材和分生组织中的水运动。现在可获得的图像分辨率约为15 μ m。首先，我们研究了这种新技术如何在植物研究领域进行新的调查。其次，重点研究了豇豆和大豆根系在土壤中的蓄水功能。最后，我们试着取水 ...更多信息 活体植物CT图像。在豇豆的情况下，这是一个非常重要的作物在干旱地区在亚洲和非洲，我们发现茎之间的第一叶和第一个三叶储存的水，当干处理进行这种水供应到其他植物组织，以支持植物的活动。用中子束研究了茎中水分的运动，用PET证实了茎的贮水功能的维持，在根中，离根1mm范围内的水分运动反映了根的活力。钒在土壤中埋置后，虽然从生长上看无明显变化，但根系活力受到严重损害。由于没有根系附近水分运动的资料，我们可以讨论根系的吸水模型。我们还模拟了土壤中水分运动对植物生长和活动的影响。为了建立该装置，我们研究了中子荧光转换器和图像处理软件的特点，特别是如何消除CCD相机中经常出现的白色斑点。将样品旋转180度，每旋转1度，得到水的图像。通过对CT图像的处理和叠加，可以得到茶花中水分的三维图像。我们现在正在根周围构建水的图像。少

Analysis of biological activity in intact cells or tissues is essential to understand information processing in plant. Techniques for these in vivo measuremerts have not been well developed, espeicially for roots. We studied a nondestructive method to image water in living plants to know information processing in plant using a neutron beam, as well as positron emitting nuclides to perform PET (Positron Emission Tomography. Neutron beam technique provided the highest resolution for water intissue yet ob tainable. With high specificity to water this neutonbeam technique images water movement in seeds or in roots imbedded in soil, as well as in wood and meristems during development. The resolution of the image attainable now is about 15 um.First, we studied how this new technique allow new investigations in the field of plant research. Next, we focused on the identification of water storing function of cowpea and soybean root activity imbedded in soil. Then finally, we tried to get water … More CT image of livingplant. In the case of cowpea, which is a very important crop at dryarea in Asia and Africa, we found the stem between the first leaf and the first trifoliatestored the water and when drytreatment was perfomed this water was supplied to the other plant tissue to support the activity of the plant. Water movement in this stem was studied by neutronbeam and the maintenance of the water storage function was confirmed by PET.In the case of root, water movoment near the root, within lmmapart from the root was found to reflect the rootactivity. When vanadium was imbedded in soil, though from the growth no observablechange was shoen, the root activity was severely damaged. Since there has been no data about water movement in the vicinity of the root, we could discuss about the water absorbing model of the root. We also simulated the plant activity and growth from water movement in the soil.To get CT image using neutron beam, we used a camation flower as an example. To set up the devise, we investigated the characteristics of neutron fluorescence converter and image processing software, espeically how to eliminate the white spots which always appear through CCD camera. We rotated the sample until 180 degree and every ldegree, we got water image. Through construsting the CT images and piled up them, three dimensional image of water in camation flower was able to get. We are now constructing the water image around the root. Less