Analysis of genomic reprogramming in mammals by utilizing inactive X chromosome.

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One of the sex chromosome, X chromosome, changes its chromatin structure during genomic reprogramming in mammalian females. We produced a construct that intensely labels inactive X chromosome by expressing GFP protein fused with MacroH2A that localizes abundantly on inactive X chromosome. It was able to trace the chromatin structure of X chromosome in culture cells and mouse early embryos by utilizing the construct. Our system would enable us to examine the process of genomic reprogramming in germ-line cells and oocytes.