Establishement of genetic resource of plants against multispectrum disease by using peptide sequences

在这个项目中，我们的工作发展如下：我们建立了一项技术，增加了几个预防功能，如去珠被、复制、细胞或身体之间的移动，以及病毒蛋白的成熟。首先，我们培育出了马铃薯Y病毒、赤豆花叶病毒、烟草蚀刻病毒、柑橘碎叶病毒以及马铃薯T病毒的运动蛋白和外壳蛋白的植株，并对它们的功能进行了分析。在此基础上，我们获得了柑橘碎叶病毒基因组的全长cDNA克隆，合成了具有侵染性的转录RNA，并建立了通过接种侵染植物的系统。在此基础上，我们对该基因进行了突变，研究了其对蛋白质功能的影响，获得了大量的信息。因此，未来有可能获得有意义的知识，将各种蛋白质应用于植物，培育出具有多光谱抗性的植物。

In this project, we have developed our work as follows :We have established the technique to add several preventing functions such as de-integument, copying, moving between cells or a body, and maturity of virus protein. Further, we have tried to produce a plant which has the newest resistant properties beyond genus of virus, and establish genetic resource of plants against multispectrum disease.First, we have produced plants which have potato virus Y,azuki bean mosaic virus, tobacco etch virus, citrus tatter leaf virus, and movement protein and coat protein of potato virus T.Then we have analyzed the functions of each protein. Proceedingly, we have produced full length of cDNA clones of citrus tatter leaf virus genome, composed infectious transcribing RNA and established a system to infect plants by inoculation. Under these results, we have introduced a mutation to the cDNA,investigated influences to the function of the protein, and got a lot of informations. Therefore, it would be possible to get significant knowledges to apply various protein to plants and produce plants which have multispectrum resistant properties in the future.