The recontsruction of periodontal ligament on artificial dental roots

1.1)与牙龈成纤维细胞和成骨细胞不同，来自牙周韧带的成纤维细胞与外周血淋巴细胞/单核细胞共培养时，可抑制破骨细胞样细胞的出现。2)研究了克隆成纤维细胞在人牙周韧带外植体培养中的生长分化（ALPase活性）。它们的个体克隆具有不同的特征。3)人牙周膜外植体附着于底物后，用无血清MCDB153培养基培养，观察到抗细胞角蛋白抗体免疫阳性的上皮细胞形态如铺路石。它们的生长和分化与人类牙龈不同。牙周韧带形成过程中的细胞外基质1)牙周韧带形成过程中纤维成纤维细胞ALPase活性与细胞、纤维和Hertwig上皮根鞘形态相关。2)形成骨质的成骨水泥细胞抗骨钙素抗体阳性，成骨细胞和成牙釉质细胞阳性。3)低钙化细胞骨质中出现抗糖胺聚糖抗体反应，并随年龄变化而改变。牙周韧带各细胞功能1)牙周韧带成纤维细胞表现ACPase活性，抗f4 /80和MOMA-2抗体阳性反应，并随咬合功能的改变而改变。2)利用免疫组织化学方法，对马拉塞氏菌体内上皮细胞的形态特征和分布进行了研究。活体染色法显示上皮细胞与成骨质细胞分布相同，且位于合成大量骨质的区域附近，参与骨质形成和合成骨质。此外，在这些上皮细胞中，未观察到BrdU的掺入，但通过免疫组织化学方法观察到作为凋亡抑制因子之一的bcl-2蛋白的反应。3)牙骨质细胞参与了牙骨质的二次矿化和牙周韧带宽度的动态平衡。少

1.Cultured cells1) The fibroblasts derived from periodontal ligament suppressed the apparence of osteoclast-like cells when co-cultured with peripheral blood lymphocytes/monocytes unlike fibroblasts from gingiva and osteoblasts.2) Growth and differentiation (ALPase activity) of the cloned fibroblasts in explant cultures of human periodontal ligament were studied. Their individual clones had various characterizations.3) When the explants of human periodontal ligament were cultured by serum-free MCDB153 medium after attachment to the substrate, the epithelial cells that were immuno-positive for the anti-cytokeratin antibody were observed like as pave stone in morphology. Their growth and differentiation were different from those of human gingiva.2.Extracellular matrices during the formation of periodontal ligament1) There was a correlation between ALPase activity of fibroflasts and the morphology of cells, fibers and Hertwig's epithelial root sheath during the formation of periodontal li … More gament.2) Cementoblasts that were forming cementum showed positive reaction of anti-osteocalcin antibody as well as osteoblasts and odontoblasts.3) The reaction of anti-glycosaminoglycans antibody was shown in the low calcified cellular cementum and it was changed by aging.3.Functions of various cells in periodontal ligament1) Fibroblasts in periodontal ligament showed ACPase activity and the positive reaction of anti-F4/80 and MOMA-2 antibody and they were changed by the occlusal function.2) Morphological charateristics and distribution of epithelial rests of Malassez were elucidated by immunohistochemical methods in vivo. The epithelial cells were demonstrated the same distribution of cementoblasts and located near the area synthesized much quantities of cementum by vital staining method, and they would participate in cementogenesis and synthesizing cementum. Furthermore, in these epithelial cells, the incorporation of BrdU was not observed, but the reaction of bcl-2 protein considered as one of the suppressors of appoptosis was observed by immunohistochemical methods.3) Cementocytes were suggested to participate in secondaly mineralization of cellular cementum and homeostasis of the width of periodontal ligament. Less