ADVENTITIOUS BUD FORMATION FROM INTERCALARY MERISTEM AND MASS PROPAGATION OF HERBACEOUS PLANTS.

由于节节组织具有产生腋芽和不定芽的能力，因此可以利用节节组织进行草本植物的大量繁殖。这里得到的方法非常简单方便。即茎尖在含0、0.1和1ppm BA的MS培养基上培养2-3周。带节的茎横切或纵切，如果茎不伸长。切片在相同培养基上继代培养，2-3周后，以相同的方式切割。从理论上讲，一个茎尖在一年的时间里可以通过重复的茎切分繁殖100多万个芽。培养温度25℃，光照条件4000勒克斯。,分别。用上述方法成功繁殖了几种草本观赏植物。培养3-4周后，除半人马花（centauria）的生根效果优于MS培养基外，其余均在含0、0.1和1ppm IBA的MS培养基上生根。将有根植株从试管中取出，在20℃和4000勒克斯光照条件下驯化2-3周。然后把它们种在温室里的土壤里。七十至夜间植株开始生长，大部分植株正常开花。因此，BA和IBA的三种浓度（0、0.1和1ppm）测试是非常有效的，因为它们涵盖了大多数草本植物的最佳生长条件。由此可见，本方法可有效地实现草本植物的节芽分离培养。

Mass propagationof of herbaceous plants was coducted using nodal tissue because the tissue has capacity to produce axillary and adventitious buds. The methology btained here was very simple and convinient. Namely, shoot apices was cultured on MS medium containing 0,0.1 and 1 ppm BA for 2-3 weeks. Then stem with nodes was traversely cut or vertically if stem did not elongate. The cut pieces were subcultured on the same media and after 2-3 weeks, they were cut in the same manner. Thoreticallly one shoot apex could be multiplied more than one million shoots by repeating shoot sectioning for one year. Culture temperature and light condition were 25C and 4000 lux., respectively. Several herbaceous ornamentals were successfully propagated with the above methods. Roots were obtained on MS medium containing 0,0.1 and 1 ppm IBA after 3-4 week culture except Centaurea for which hyponex nedium was better than MS medium. Rooted plants were taken out from test tubes and acclimatized at 20C and 4000 lux light conditions for 2-3 weeks. They were then planted in the soil in the greenhouse. Seventy to nighty plants started growth and most of plants flowere normally. Thus three concentrations (0,0.1 and 1 ppm) test of BA and IBA were very effective because they covered optimum growth conditions for most of herbaceous plants. In conclusion herbaceous plants could be effectively propagated by the node and shoot split culture by our method.