Relationship among blood pressure, tail blood flow and plasma catecholamine levels in chronic spinal rats.

在慢性脊髓损伤大鼠，观察了体循环血压（BP）、尾血流量与血浆儿茶酚胺、去甲肾上腺素（NA）和肾上腺素（AD）的关系。在下颈部切断成年Wistar京都大鼠（WKY）的脊髓，并将大鼠作为慢性脊髓大鼠饲养在温暖的房间（30 ℃）中。采用尾袖法测量体循环血压（BP），其余病例采用股动脉压力传感器测量BP。通过心电图R-R间期计算心率（HR），在清醒状态下经颈静脉插管采血。结果：A：急性脊髓化的影响：在Th 1（A组）或C7（B组）水平横断脊髓。在A组大鼠中，Th 1脊神经保持完整，其包含心脏交感神经纤维（Waibl H，1973）。在A组中，急性脊髓化引起HR和CO2持续增加， ...更多信息 针刺后体温逐渐升高，血压略有下降。仅A组大鼠在脊髓化后存活。B：尾部血管的药理学研究。在慢性脊髓大鼠中保持中间状态（不收缩但不完全扩张）。普萘洛尔（β-肾上腺素能拮抗剂，8 mg/kg）或异丙肾上腺素（β-肾上腺素能激动剂，0.1 mg/kg）诱导了短暂的尾部血管收缩。C：血浆NA和AD水平：脊髓横断后，全身血压从120 mmHg降至80 mmHg。血压逐渐恢复，但未达到奥斯本报告的正常值水平（美国生理学杂志，1989）。脊髓化后第1周NA水平（平均<plus-minus>S.E）为50 <plus-minus>32 pg/ml（N=6），第2周为147 <plus-minus>42 pg/ml（N=7），第2周显著高于第1周（P&lt;0.0 5），第1周AD为594 <plus-minus>32 4 pg/ml，第2周为30 9 <plus-minus>15 3 pg/ml，两组间无显著性差异。提示肾上腺髓质等体液因子在慢性肺心病血管调节中起重要作用

In chronic spinal rats, relationship among the systemic blood pressure (BP), the tail blood flow and plasma catecholamines, noradrenaline (NA) and adrenaline (AD). Spinal cord of adult Wistar Kyoto rat (WKY) was transected at lower cervical portion, and the rats were reared as chronic spinal rats in a warm room (30ﾟC). The systemic blood pressure (BP) was measured by the tail cuff method, in other cases BP was measure by a pressure transducer at the femoral artery. Heart rate (HR) was calculated by of R-R interval of a ECG.Blood sample was drawn through the cannula of the jugular vein under conscious condition. Plasma level of NA and AD were estimated by HPLC.RESULTS : A : Effect of the acute spinalization : Spinal cord was transected at level of Th1 (Group-A) or C7 (Group-B) of the vertebral segment. In Group-A rats, Th1 spinal nerve remain intact which contain the cardiac sympathetic nerve fibers (Waibl H,1973). In Group-A,acute spinalization induced a sustained increase of HR and co … More re temperature gradually increased after the spinalization, BP decreased slightly. Only Group-A rats survived after the spinaliztion. B : Pharmacological studies of blood vessels of the tail. Intermediate condition (not constricted but not fully dilated) was kept in chronic spinal rats. Either propranolol (beta-adrenergic antagonist, 8 mg/kg) or isoproterenol (beta-adrenergic agonist, 0.1 mg/kg) induced a transient tail vasoconstriction. C : plasma level of NA and AD : By the spinal transection, systemic BP was decreased from 120 mmHg to 80 mmHg. BP recovered gradually day by day but did not attained level of normal value as reported by Osborn (Am J Physiol, 1989). Mean NA level (mean <plus-minus> S.E) was 50<plus-minus>32 pg/ml (N=6) in the 1st week (W) after the spinalization, and 147<plus-minus>42 pg/ml (N=7) in the 2nd W.Value in the 2nd W was significantly (P<0.05) high compared to value of 1st W.Mean AD was 594<plus-minus>324 pg/ml in the 1st W and 309<plus-minus>153 pg/ml in the 2nd W,there was no significant difference between these values. From these results, it is suggested that humoral factors from adrenal medulla and others are more important for the regulation of the blood vessels in chronic sp Less