Immunological and molecular biological study in occupational, environmental and community health

在这项研究中，我们测量微小淋巴细胞亚群使用双色染色的主题相关的领域的职业和环境医学和社区医学。另外，我们尝试将分子生物学应用于解决职业医学中的问题。结果发现：1.芳香胺接触者外周血中CD_4 ~+ CD_（45）RA ~+T细胞选择性降低。另一方面，我们发现这些工人的CD 57 + CD 16自然杀伤（NK）细胞增加。2.吸烟者的CD 4 + CD 29 +T细胞选择性增加。我们发现（1）吸烟者的CD 4 +T细胞增加，（2）铬酸盐对CD 4 +T细胞和CD 8 + T细胞的影响降低。接触铬酸盐的工人CD 4 +T细胞和CD 8 +T细胞均降低。此外，铬盐作业工人的CD 4 + CD 45 RA +T细胞和CD 57 + CD 16 +NK细胞（具有中等的NK活性）也明显降低。5.在校正年龄和吸烟的影响后，铅作业工人的CD 3 + CD 45 RO+（记忆）T细胞明显降低。2名高铅作业工人注射Ca-EDTA 10（6）周后IgG水平升高，提示其抗体生成功能得到改善。6.朝鲜族高汞作业工人CD 4 + CD 45 RA +T细胞明显下降。应用SSCP和测序方法，对7例石棉恶性间皮瘤原发灶DNA进行了H-ras和p53基因的检测。

In this study, we measured minute lymphocyte subpopulations using two-color staining in subjects relevant to the fields of occupational and environmental medicine and community medicine. In addition, we tried to apply the molecular biology for solving the problems in occupational medicine. We obtained the following findings.1.We found a selective decrease in CD4+CD45RA+T cells in workers who had been exposed to aromatic amines. On the other hand, we found an increase in CD57+CD16 natural killer (NK) cells in these workers.2.A selective increase in CD4+CD29+T cells was found in smokers.3.By the analyzes controlling for the effects of smoking, we found (1) an increase in CD4+T cells in smokers and (2) the decreasing effects of chromates on CD4+T and CD8+T cells were stronger than those of aromatic amines.4.Both CD4+T and CD8+T cells were decreased in workers who had been exposed to chromates. Moreover, decreases in CD4+CD45RA+T cells and CD57+CD16+NK cells (with intermediate NK activity) were found in chromate workers.5.A significant decrease in CD3+CD45RO+(memory) T cells were found in lead workers after adjusting for the effects of age and smoking. We found the elevated IgG in 2 workers exposed to high level of lead after 10 (6) week injections of Ca-EDTA,suggesting the improved functions of antibody production.6.A significant decrease in CD4+CD45RA+T cells was found in Korean workers exposed to high level of mercury.7.We found no mutations in K-ras, H-ras and p53 genes in extracted DNA from samples of the primary tumors of malignant mesothelioma in 7 asbestos patients by the SSCP and sequencing methods.