Study on mechanisms of outgrow in bronchial asthma

为了探讨支气管哮喘的发病机制，我们研究了（1）用流式细胞术检测细胞内产生的细胞因子的新系统，研究了活动期哮喘患者和哮喘缓解期患者的尘螨变应原刺激的T细胞产生的细胞因子，（2）过敏原特异性免疫治疗前后从外周血单个核细胞建立的屋尘螨特异性T细胞系的细胞因子产生。流式细胞仪检测结果显示，活动期哮喘患者经尘螨变应原刺激后，外周血CD 4 + T淋巴细胞产生IL-4和IL-5的水平明显高于正常对照组。在免疫治疗前建立的屋尘螨特异性T细胞系产生的IL-4和IL-5的量大于免疫治疗后从相同患者建立的T细胞系。这些结果表明，减少Th 2细胞因子，如IL-4和IL-5的屋尘螨反应性T细胞可能与支气管哮喘的自然和治疗为基础的产物。

In order to investigate the mechanisms of outgrow of bronchial asthma, we studied (1) cytokine production of house dust mite allergen-stimulated T cells either in patients with active asthma or in patients who outgrew from asthma by newly developed system for detection of intracellularly produced cytokine by use of flow cytometry, (2) cytokine production of house dust mite-specific T cell lines established from peripheral blood mononuclear cells before and after allergen-specific immunotherapy. Flow cytometrical analysis revealed that peripheral CD4_+ T lymphocytes in active asthma patients stimulated with house dust mite allergen produced much higher amounts of interleukin 4 (IL-4) and IL-5 than those in outgrown patients. House dust mite-specific T cell lines established before immunotherapy produced greater amounts of IL-4 and IL-5 than those established from the same patients after immunotherapy. These results indicate that reduction of Th2 cytokines such as IL-4 and IL-5 from house dust mite-reactive T cells may be related to outgrow of bronchial asthma both in spontaneous and therapy-based outgrow.