THE STUDY OF ANGIOGENETIC FACTOR GENE EXPRESSION IN HEPATOCELLULAR CARCINOMA

应用北方杂交和逆转录-聚合酶链反应（RT-PCR）检测18例肝细胞癌（HCC）组织中血管内皮生长因子（VEGF）、碱性成纤维细胞生长因子（bFGF）和血管生成素（angiogenin）的转录表达。研究了信使RNA（mRNA）表达与动脉造影结果以及组织病理学结果的相关性。免疫组化法检测VEGF、bFGF和血管生成素在HCC组织中的表达，VEGF、bFGF和血管生成素mRNA在HCC组织中的表达分别为10例、7例和3例。这些血管生成因子mRNA表达的程度与血管造影中肿瘤染色强度、肿瘤大小和恶性组织学分级相关。免疫组化结果显示，VEGF和bFGF蛋白在HCC细胞中呈强阳性表达。相反，在血管生成素蛋白中没有观察到差异。总之，这些结果表明，VEGF和bFGF有助于HCC的血管生成和肿瘤生长。

Northern blot analysis and reverse transcription-polymerase chain reaction (RT-PCR) were used to examine the transcriptional expression of vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), and angiogenin in 18 hepatocellular carcinoma (HCC). Correlations between messenger RNA (mRNA) expression and arteriographic findings, as well ashistopathological findings, were studied. Immunohistochemistry was performed to identify the localization of cells expressing VEGF,bFGF,and angiogenin in HCC.Higher levels of VEGF,bFGF,and angiogenin mRNA were observed in 10 cases, 7 cases, 3 cases than in corresponding nontumorous tissues, respectively. The degree of these angiogenetic factor mRNA expression were correlated with the intensity of tumor staining in angiograms, the tumor size, and the histological grade of malignancy. On immunohistochemical obsevation, VEGF and bFGF protein were intensely detected in HCC cells. In contrast, no difference was observed in angiogenin protein. In conclusion, these results suggest that VEGF and bFGF contribute to angiogenesis and tumor growth of HCC.