Studies on the pathogenesis of early lesions in Crohn's disease : Clinical and experimental investigation

1. 临床研究为了阐明结肠淋巴样结节的滤泡相关上皮（FAE）在克罗恩病溃疡形成中的作用，我们通过放大结肠镜、电镜和免疫组化检查对10例克罗恩病患者进行了研究。结果，在扫描电镜下，放大结肠镜下未见明显糜烂的淋巴样结节被红色晕包围，表面糜烂大小为150-120mum。扫描电镜和透射电镜的相关性显示，在侵蚀的边缘有包括M细胞在内的FAE残留物。免疫组织化学研究发现，在克罗恩病活动期，HLA-DR抗原在整个炎症结肠粘膜中的表达比在缓解期更强烈。因此，淋巴滤泡周围的红色光晕似乎预示着可见的扁桃体溃疡，提示克罗恩病的溃疡起源于fae。短句来源为了进一步研究吲哚美辛诱导小肠损伤后隐窝增殖和生长因子表达的早期变化，采用扫描电镜（扫描电镜）、隐窝增殖（采用Brd U免疫组化染色）和肠道tgf - β _1、IGF-1、HGF和NGF mRNA表达（RT-PCR）对24 mg/kg吲哚美辛给予大鼠进行了研究。扫描电镜显示，在1小时内，中小肠绒毛扭曲扭曲。2小时后，小肠中隐窝的增殖没有改变，而5小时后，所有部位的隐窝增殖都有所减少。IGF-1和HGF在6小时时表达明显，而NGF在此时间点未检测到。这些结果提示，吲哚美辛诱导的早期粘膜损伤以隐窝结构改变为特征，但未改变隐窝细胞的增殖，某些生长因子的改变可能在粘膜损伤的进展中起作用。少

1. Clinical investigationTo clarify the role of the follicle associated epithelium (FAE) of colonic lymphoid nodules in the formation of ulcers in Crohn's disease, 10 patients with this disease were studied by magnifying colonoscopy, electron microscopy, and immunohitochemical examination. As a result, scanning electron microscopy of lymphoid nodules surrounded by a red halo without visible erosions by magnifying colonoscopy showed surface erosions 150-120mum in size. Correlation of scanning and transmission electron microscopy revealed residues of FAE including M cells at the edges of the erosions. In immunohistochemical studies, HLA-DR antigen was more strongly expressed in the entire inflamed colonic mucosa in the active stage of Crohn's disease than in the remission stage. Therefore, the red halo appearance surrounding lymphoid follicles seems to preced visible aphthoid ulcers and suggests that ulcerations in Crohn's disease originate from FAE.2. Experimental investigationTo charac … More teriza early changes in both cryptal proliferation and expression of growth factors in indomethacin-induced small intestinal damage, scanning electron microscopic findings, cryptal proliferation (using immunohistochemical staining for Brd U), and intestinal mRNA expression of TGF-beta_1, IGF-1, HGF,and NGF (using RT-PCR) were investigated in rats which were received 24 mg/kg of intracolonic indamethacin. Scanning electron microscopy showed that the villi in the mid-small intestine was distorted with tortuous fashion at an hour. Cryptal proliferation in the mid-small intestine was not altered at 2 hour whereas it was decreased at all sites after 5 hours. IGF-1 and HGF expression became obvious at 6 hour, while NGF could not be detected at this time point. These findings suggest that early mucosal damage induced by indomethacin is characterized by alteration of the crypt architecture without changes in crypt cell proliferation, and that changes of some growth factors may play a role in the progression of the mucosal damage. Less