Synthesis and Structure-Function Relationship Study of Pore Structures emulated Ligand-gated Ionic Channel

(1)合成了具有预测的肉毒杆菌神经6xin A跨膜序列的肽。单通道记录数据表明，它在脂质双层中形成阳离子选择性离子通道。(2)采用新的化学选择性连接方法合成了几个模拟乙酰胆碱受体孔结构的模板组装螺旋束蛋白。(3)从理论上研究了具有固定电荷和偶极子的软极性界面膜的离子渗透性。(4)合成了具有预测的甲型流感病毒M2蛋白跨膜序列的肽段，并研究了其在双层膜中的结构和质子转运功能。(5)采用单通道记录法研究了共离子对gramicidin A通道质子电导的影响，并用理论模型对结果进行了分析。(6)利用x射线衍射和荧光光谱技术研究了六方凝胶相和交叉指状凝胶相等生物膜非双层结构的稳定性和结构。这些结果表明，膜表面与溶剂之间的相互作用自由能对膜的稳定性起着重要的作用。(7)研究了F-actin组装的多态性，并绘制了其定量相图。

(1)A peptide with the predicted transmembrane sequence of botulinum neurot6xin A was synthesized. Single-channel recording data shows that it forms cation-selective ionic channels in lipid bilayers.(2)A few template-assembled helix bundle proteins emulated the pore structure of acetyicholine receptor were synthesized by a new chemoselective-ligation method developed by us.(3)Ion permeability of a membrane with soft polar interfaces with fixed charges and dipoles were investigated theoretically.(4)A peptide with the predicted transmembrane sequence of M2 proteins of influenza virus A was synthesized, and its structure in bilayer membranes and function of proton transport were studied.(5)Effect of co-ions on proton conductance of gramicidin A channel were investigated by the single channel recording method, and results were analyzed by a theoretical model.(6)Stability and structure of non-bilayer structures of biomembranes such as hexagonal II and interdigitated gel phases were studied by X-ray diffraction and fluorescence spectroscopy. These results indicate that interaction free energy between segments of membrane surface and solvents plays an important role in the stability of these membranes.(7)Polymorphism of F-actin assembly was studied, and its quantitative phase diagram was made.