Induction of drug induced tolerance in mice and application for large animals

根据1995年进行的分析，得出了以下结果。1)嵌合体仅在淋巴细胞水平诱导，在骨髓水平不诱导。2)B10 D2和B10（单独MHC）联合应用，可观察到克隆性破坏，但嵌合体的诱导是短暂的; 3）皮肤移植物存活时间的延长与嵌合状态的持续时间有显著关系。我们用白消安与我们传统的CP诱导耐受方案相结合，观察到了永久性嵌合体和皮肤移植存活，这是令人鼓舞的数据，我们希望达到的最终目标是在大型动物中仅通过药物诱导耐受。我们认为我们的新数据将取得更大的成功。

From analysis performed in 1995, the following results were obtained. 1) Chimerism was induced only in lymphocytes, and wasn't induced in bone marrow level. 2) In the combination of B10D2 and B10 (MHC alone disparate), clonal destruction was observed, but the induction of chimerism was transient, and 3) skin graft survival prolongation and duration of chimeric state had significnat relationship.From 1996, we started the analysis of our new protochol (SC+CP+Busulfan+Bone marrow cells protochol) to break through the MHC obstacle. We got very encouraging data that permanent chimerism and skingraft survaival were observed by using busulfan with our traditional CP induced tolorance protochol.The final goal that we want to achive is to induce tolerance only by drugs in large animals. And we think our new data is going to be a greater success.