Study of assisted liver transplantation for acute liver failure -Development of xeno auxiliary liver transplantation-

为探索急性和亚急性肝功能衰竭的生命支持方法，我们开展了动物辅助性异位肝移植（ALT）的研究，首次在大鼠肝外门静脉阻塞模型上进行了ICG清除剂（RK 1,000）无创性的实验研究。这个工具是非常有用的，特别是在小动物，用于评价肝功能没有采样的外周blood.In本项目的第二部分，一个简单的ALT模型建立在大鼠使用袖带应用。在先天性高胆红素血症古恩大鼠中测试了正常肝脏短暂停车的效果。古恩大鼠与Wistar大鼠的正常肝脏异位移植后，血清中的阿珠宾浓度显著降低并维持在正常水平。移植后1个月安全地进行了移植物切除术，但古恩大鼠的总胆红素水平没有恢复到术前值。ALT肝内可能含有肝干细胞并分化为产酶细胞。最后，从小鼠或仓鼠到大鼠进行了异种ALT实验。胆道重建困难。环磷酰胺联合FK-506治疗可有效延长移植物存活时间。

We studied auxiliary heterotopic liver transplantation (ALT) in animals to develop a life support method for acute and subacute liver failure.An experimental study on a rat model of extrahepatic portal obstruction was firstly performed to evaluate non-invasive method of ICG clearance mater (RK 1,000). This tool was very useful, especially in small animals, for evaluation of liver function without sampling of peripheral blood.In the second part of this project, a simple ALT model was established in rats using the cuff application. Effects of transient parking of normal liver were tested in congenitally hyperbilirubinemic Gunn rats. Serum bilirubine concentrations of Gunn rats, transplanted heterotopically with normal livers of Wistar rats, were dramatically reduced and maintained to the normal levels. The graftectomy was performed safely 1 month after transplantation, but total bilirubine levels were not returned to the preoperative value of Gunn rats. There was a possibility that hepatic stem cells were included in ALT liver and generated to enzyme producing cells.Finally, xenogenic ALT was performed from mice or hamsters to rats. A difficulty was found in reconstration of biliary tract. The combination therapy of cyclosphamide plus FK-506 was effective for prolongation of the graft survival.