脳血管攣縮の平滑筋細胞障害におけるアポトーシスの関与とサイトカインの効果の研究

测定犬基底动脉细胞内钙水平，以研究其平滑肌Ca2+调节是否在蛛网膜下腔出血后慢性血管痉挛期间发生改变。采用双出血模型。初次出血后7天血管造影证实血管痉挛的发生。用Fura-2测定平滑肌细胞内钙浓度（[Ca2+] i）。以R340/356比值作为[Ca2+] i的指标。当胞外钙浓度（[Ca2+] e）从pCa 8增加到pCa 2时，[Ca2+] i也随之增加。在痉挛动脉中，与正常动脉相比，[Ca2+] e- [Ca2+] i曲线升高。在正常组中，离子霉素使曲线升高，但在痉挛动脉中效果不明显。[Ca2+] i的值（以Kd的倍数计算）在痉挛动脉中更大。地尔硫卓（10-5 mol/l）部分抑制痉挛动脉中增强的[Ca2+] i信号，而对对照组的曲线没有影响。提示蛛网膜下腔出血后平滑肌钙调节功能受损，可能参与慢性血管痉挛的发病机制。

The intracellular calcium level was determined in the canine basilar artery in order to investigate whether Ca2+ regulation of its smooth muscle is altered during chronic vasospasm following subarachnoid hemorrhage. A double hemorrhage model was used. The occurrence of vasospasm was confirmed angiographically seven days after initial hemorrhage. The intracellular calcium concentration ( [Ca2+] i) of smooth muscle was measured using Fura-2. Fluorescence to excitation at 340 and 356 nm was monitored and the ratio R340/356 was used as the indicator of [Ca2+] i. When the extracellular calcium concentration ( [Ca2+] e) was increased from pCa 8 to 2, [Ca2+] i also increased. In the spastic arteries, the [Ca2+] e- [Ca2+] i curve was elevated as compared to the normal rteries. Treatment with ionomycin elevated the curve in the normal group, but it had little effect in the spastic arteries. Values of [Ca2+] i, calculated in multiples of Kd, were greater in the spastic arteries. Diltiazem (10-5 mol/l) partially suppressed the augmented [Ca2+] i signal in the spastic arteries, whereas it did not affect the curve in the control group. These results indicate that calcium regulation of smooth muscle impaired after subarachnoid hemorrhage, which may contribute to the pathogenesis of chronic vasospasm.