A STUDY ON ANTI-INFLAMMATORY ACTION OF A NEW ENDOGENOUS OPIOIDE-REGULATED SUBSTANCE

多酚是一种内源性的疼痛调节物质，它调节脑啡肽的降解活性。在本研究中，我们发现Spinogin对FMLP刺激的人PMN的炎症反应有明显的抑制作用，可能是一种新的内源性抗炎肽，其部分机制是通过抑制[Ca^&lt；2+&gt；]i的变化来实现的。接下来，我们使用小鼠气囊作为急性炎症反应的模型，研究了旋诺芬的作用。在该模型中，炎症部位聚集的角叉菜胶和中性粒细胞被用作炎症诱导剂，炎症部位聚集的中性粒细胞是急性炎症反应的指示物。角叉菜胶诱导的气囊部位静脉注射旋吗啡，与生理盐水组比较，抑制率为%。联合应用亮氨酸和氨基肽酶N抑制剂可显著抑制角叉菜胶诱导的中性粒细胞积聚。在未来，Spinogin将成为阐明神经肽诱导的生物现象(如脑啡肽介导的疼痛和炎症)的重要工具。

Spinorphin is an endogenous pain-regulated substance which modulated an enkephalin-degrading activity. In this study, we found that Spinorphin causes a significant inhibition of inflammatory responses by human PMNs stimulated with FMLP and may be a new endogenous anti-inflammatory peptide which is mediated in part through the suppression in [Ca^<2+>]i changes. Next we examined the effects of Spinorphin, using an in vivo system involving mouse air-pouch as a model of acute inflammatory response. In this model carrageenan and PMNs accumulation in inflammatory sites were used as a inflammation-tiggerring agent and PMNs accumulation at inflammatory sites was an indicator of an acute inflammatory response. Intravenous administration of Spinorphin within air-pouch site elicited by carrageenan, the inhibition being 64%, compared to the saline-treated group. Combination of Leuhistin, and inhibitor of aminopeptidase N,sinergestically suppressed PMNs accumulation induced carrageenan. In the future, Spinorphin will be an important tool for use in clarifying of neuropeptide-induced biological phenomena such as enkephalin mediated pain and inflammation.