Positive Control of Gonadotropin Secretion by Androgen

我们在单细胞水平上研究了睾酮（T）对雄性大鼠垂体前叶细胞分泌FSH的影响。将垂体前叶细胞与10 ng/ml T共同培养72 h后，进行单分散培养，用细胞免疫印迹法检测FSH，用显微图像分析仪对FSH特异性细胞印迹进行定量。FSH分泌细胞的数量检测到的免疫反应细胞印迹上的转移膜增加到6小时的孵育，并表示4.1%的总垂体细胞上的膜在6小时的孵育。单个细胞分泌FSH的量从小于20到大于8，000 fg/cell/h不等。单个细胞的平均FSH分泌量以孵育时间依赖性方式增加，直至6小时。在孵育的任何时间，向培养基中加入10 ng/ml的T均未改变FSH分泌细胞的数量。T给药增加了64%的平均FSH分泌后，3小时的孵育，导致从单细胞的FSH分泌的频率分布向右移动。FSH的总量，即每个FSH分泌细胞分泌的FSH的总和，通过添加T增加了92%，并且这种增加的83.7%可归因于最大分泌子亚组的FSH分泌增加（大于5 pg/细胞/3 h）。然而，FSH分泌量的前10名的最大的分泌亚组的平均值没有显着差异控制和T-治疗groups.The本研究分析了第一次从大鼠垂体前叶细胞在单细胞水平上的FSH分泌。结果表明，T刺激FSH分泌在体外不是由于FSH分泌细胞的数量增加，但由于FSH分泌从每个细胞的增加。

We investigated the effects of testosterone (T) on FSH secretion from male rat anterior pituitary cells in cultures at the single cell level. Anterior pituitary cells cultured with or without 10 ng/ml T for 72 h were monodispersed and subjected to cell immunoblot assays for FSH.Cell blots specific for FSH were quantified by means of a microscopic image analyzer. The number of FSH-secreting cells detected as immunoreactive cell blots on the transfer membrane was increased up to 6 h incubation and represented 4.1% of total pituitary cells applied on the membrane at 6 h of incubation. The amount of FSH secreted by single cells varied from less than 20 to greater than 8,000 fg/cell/h. The mean FSH secretion from individual cells was increased in an incubation time-dependent manner up to 6 h. The number of FSH-secreting cells was not changed by the addition of 10 ng/ml of T into the culture medium at any time of incubation. T administration increased the mean FSH secretion by 64% after 3 h incubation, resulting in a shift to right in frequency distribution of FSH secretion from single cells. The total amount of FSH namely the sum of FSH secreted by each FSH-secreting cell was increased by 92% by the addition of T and 83.7% of this increase was attributable to an increase in FSH secretion from the largest secretor subgroup (greater than 5 pg/cell/3h). However, mean amounts of FSH secretion by the top 10 of the largest secretor subgroup were not different between control and T-treated groups.The present study analyzed for the first time FSH secretion from rat anterior pituitary cells at the single cell level. The results suggest that stimulation by T of FSH secretion in vitro is not due to the increase in the number of FSH-secreting cells but due to the increase in FSH secretion from each cell.