Prevalence of Human Papillomavirus in Cervical Cancer in Okinawa

1)人乳头瘤病毒（HPV）在冲绳宫颈癌筛查涂片试验中的患病率HPV感染现已被证明是大多数宫颈上皮内瘤变病例的主要原因，并且是大多数宫颈癌病例的必要原因，但不是充分原因。HPV检测依赖于剥离细胞标本中病毒脱氧核糖核酸（DNA）的检测。我们通过聚合酶链反应（PCR）检测了宫颈发育不良的进展与HPV患病率之间的相关性。使用L1一致引物的PCR至少可以鉴定出6、11、16、18、31、33、42、52和58这9种HPV类型(6)，我们的研究还需要详细的HPV分型。从冲绳接受宫颈癌涂片检查的3,985名妇女中收集了宫颈刮痕。正常宫颈（宫颈ⅰ、ⅱ级）hpv患病率分别为：30岁以下18.7%（45/241）、31-40岁8.0%（47/587）、41-50岁9.3%（76/817）、51-60岁10.0%（106/1059）、61-70岁10.2%（104/1018）、70岁以上9.8%（26/263）。目前，我们正在前瞻性地研究治疗后HPV DNA的患病率以及HPV阳性对异常细胞学和宫颈上皮内瘤变（CIN）复发的预测价值。我们的研究表明HPV DNA的检出率与CIN的检出率是一致的，有效的手术治疗CIN也可以消除可检出的HPV DNA。2)宫颈癌可疑转移组织中人乳头瘤病毒检测的临床意义。人乳头瘤病毒（hpv）在宫颈癌原发疾病中普遍存在。这些研究中的绝大多数都是使用该技术检测石蜡包埋组织中的HPV dna。另一方面，只有有限的数据可用于转移性病变中HPV dna的检测。本研究的目的是建立从新鲜组织标本中提取DNA用于HPV分析的方法，并探讨HPV的存在与怀疑转移组织的病理证据之间的关系。从怀疑有转移的新鲜组织和原发性宫颈病变制备的细胞样本中均可检测到HPV dna。在所有HPV阳性患者中，转移灶的HPV型与原发宫颈病变的HPV型相同。在组织学未证实转移的6例hpv阳性淋巴结中，3例在几个月后在hpv阳性淋巴结所在的部位复发，提示在采样时淋巴结已经转移。在怀疑转移的组织中检测hpv阳性可以为评估可能的转移提供早期信息。少

1)Prevalence of human papillomavirus (HPV) in the screening smear test for cervical cancer in OkinawaHPV infection has now been shown to be the primary cause of most cases of cervical intraepithelial neoplasia and is a necessary, but not sufficient, cause of most cases of cervical cancer. HPV testing relies on the detection of viral deoxyribonucleic acid (DNA) in exfoliated cell specimens.We examined the correlation between the progression of cervical dysplasia and the prevalence of HPV by means of the polymerase chain reaction (PCR).The PCR with L1 consensus primers could identify at least nine HPV types, 6,11,16,18,31,33,42,52 and 58 (6), and the detailed typing of HPVs is needed in our study.Cervical scrapes were collected from 3,985 women who received the screening smear test for cervical cancer in Okinawa.The prevalence of HPVs in normal cervices (Pap class I and II) was that under 30 years old : 18.7% (45/241), 31-40 years old : 8.0% (47/587), 41-50yrs : 9.3% (76/817), 51-60yrs : … More 10.0% (106/1059), 61-70yrs : 10.2% (104/1018) and over 70yrs : 9.8% (26/263).At present, we are investigating, prospectively, the prevalence of HPV DNA after treatment and the predictive value of HPV positivity for recurrence of abnormal cytology and cervical intraepithelial neoplasias (CIN). Our study indicates that detectability of HPV DNA does coincide with detectability of CIN and that effective surgical treatment of CIN can also eliminated detectable presence of HPV DNA.2)Clinical significance of human papillomavirus detection from metastasis-suspected tissues in cervical cancer.Studies on Human papillomaviruses (HPVs) are prevalent in primary diseases of cervical cancer. The vast majority of these studies have been done using the technique to detect HPV DNAs in paraffin-embedded tissues. On the other hand, only limited data are available on the detection of HPV DNAs in metastatic lesions.The aim of the present study was to establish the procedure of DNA extraction from fresh tissue specimens for HPV analysis and to examine the correlation between the presence of HPVs and pathological evidence in metastasis-suspected tissues.CONCLUSION1.HPV DNAs were detectable in cell samples prepared from fresh metastasis-suspected tissues as well as primary cervical lesions.2.HPV type in metastatic lesions was identical with that in their primary cervical lesions in all of HPV-positive patients.3.Of 6 cases with HPV-positive nodes not histologically proven to be metastasized, 3 suffered from recurrence a few months later at the sites where the HPV-positive nodes were located, suggesting that the nodes had been already metastasized at the time of sampling.4.HPV-positive detection in metastasis-suspected tissues could provide early information for the appraisal of possible metastasis. Less