Glycobiological Study of Malignant Tumors in Ophthalmology

本文应用凝集素组织化学和原位杂交组织化学方法对眼睑和眼眶恶性肿瘤中的糖复合物进行了分析。我们认为，细胞，表达的ST-mRNA的细胞质分布和结合的凝集素，特异性唾液酸，细胞表面，积极生产唾液酸聚糖。我们还认为，其表面用凝集素染色而没有ST-mRNA的胞质分布的细胞已经完成了唾液酸聚糖的合成。基于这些观点，这种糖组化技术是有用的动态分析的糖缀合物在个别肿瘤细胞。结果表明，O-连接唾液酸糖链（O-linked sialoglycan）在脂溢性角化病的肿瘤中可能分布于增厚的脊髓细胞层细胞上，并参与其病理机制。用该方法对眼睑基底细胞癌和泪腺多形性腺瘤进行了分析，结果发现眼睑鳞状细胞癌和皮脂腺癌的肿瘤细胞中均检测到O-糖结合物MUC 1，而眼睑基底细胞癌和良性肿瘤的肿瘤细胞中均未检测到MUC 1。这些发现提示MUC 1可能参与了包括转移在内的恶性特征的表达。而Ki 67抗原在眼睑恶性肿瘤中的表达率明显高于良性肿瘤，提示Ki 67可作为鉴别良恶性肿瘤的指标。角蛋白的免疫组化分布显示，眼睑基底细胞癌由均匀的肿瘤细胞组成，所有肿瘤细胞均表达相同的角蛋白，而鳞状细胞癌和皮脂腺癌则由不同分化的肿瘤细胞组成。

The glycoconjugates in various malignant tumors in eyelids and orbita were analyzed by lectin histochemistry and in situ hybridization histochemistry using cRNA probes for sialiltransferases (ST). We considered that the cells, which expressed both cytoplasmic distribution of ST-mRNA and binding of lectins, specific for sialic acids, to the cell surfaces, were actively producing sialoglycans. We also considered that the cells, whose surfaces were stained with the lectins without cytoplasmic distribution of ST-mRNA,have completed the synthesis of sialoglycans. Based on these view points, this glycohistochemical technique is useful for dynamic analysis of the glycoconjugates in individual tumor cells. This technique revealed that O-linked sialoglycan, whose turnover-rate is slow, may be distributed over the cells of thickened spinocellular layr in the tumor of seborrheic keratosis and involved in its pathomechanism. We also analyzed the basal cell calcinoma in eyelids and the lacrimal pleomorphic adenoma using this method.MUC 1, one of the O-linked glycoconjugates, was immunohistochemically detected in the tumor cells of all cases of squamous cell carcinoma and sebaceous carcinoma in eyelids whereas it was absent in those of all cases of basal cell carcinoma and benigh tumors in eyelids. These findings suggested that MUC 1 may be involved in expression of malignant features including metastasis. On the other hand, the Ki 67 antigen-expression rates of malignant eyelid tumors were higher than those of benigh tumor, suggesting that Ki 67 can be used to distinguish between malignant and benigh tumors. Immunohistochemical distribution of kearatin revealed that the basal cell carcinoma in eyelids is consisted of uniform tumor cells, all of which express the same keratin, while the squamous cell carcinoma and sebaceous carcinoma are composed of various differentiatiating tumor cells.