Analysis of Cell Adhesion Molecules and Stromal Factors Associated with Infiltration and Metastasis in Oral Squamous Cell Carcinoma

1.应用免疫组化方法检测nm 23-H1蛋白在口腔腺样囊性癌（ACC）和口腔鳞癌（OSCC）中的表达，并分析其与临床病理特征的关系。nm 23-H1在ACC中的表达有一半以上降低，且在有转移和预后不良的病例中表达降低更为常见。此外，转移性肿瘤的免疫反应性下降，与他们的原发性肿瘤。结论nm 23-H1在口腔鳞癌中的表达与ACC的转移有关，但在口腔鳞癌中，尽管约有一半的病例nm 23-H1表达降低，但nm 23-H1的表达与ACC的转移频率无关。细胞膜上的CD 44 v6表达在不典型增生的上皮中已经减少，并变得不均匀。CD 44 v9在口腔鳞癌中的低表达与淋巴结转移及预后密切相关。p53、bcl-2蛋白的表达与肿瘤细胞对放射治疗的抵抗有关。TGF-β是血管生长因子之一，在口腔鳞癌间质中的成纤维细胞、巨噬细胞和血管内皮细胞中均有表达，可能作为自分泌或旁分泌系统刺激内皮细胞增殖。这一发现表明癌细胞本身对基质的破坏有积极的帮助。

1.The expression of nm23-H1 protein was evaluated immunohistochemically in adenoid cystic carcinoma (ACC) and oral squamous cell carcinoma (OSCC) and correlated with their clinicopathologic features. In ACC,more than half cases showed the reduction of nm23-H1 expression and the reduction was more commonly observed in the cases with metastasis and unfavorable prognosis. Furthermore, metastatic tumors showed decreased immunoreactivity compared with their primary tumors. These results may suggest that nm23-H1 has an implication for metastasis of ACC.In OSCC,although about half cases showed the reduced expression, there was no relationship between the frequency of metastasis and the stainability of nm23-H1.2. The expression of CD44v6 on the cytomembrane already decreased and became heterogenous in the dysplastic epithelium. The reduced expression of CD44v9 was frequently observed in OSCC and its was significantly related to lymph node metastasis and worse prognosis. The alteration of CD44 expression may have some role in metastasis.3.The accumulation of p53 and bc1-2 proteins was related to the resistance of cancer cells to radiation therapy. Apoptosis of the cancer cells induced by irradiation may partly be inhibited by these proteins in OSCC.4.TGF-beta, one of the vascular growth factors, was expressed in fibroblasts, macrophases and vascular endothelial cells in the cancer stroma and may act as autocrine or paracrine system to stimulate the proliferation of endothelial cells.5.Plasminogen activator was expressed in cancer cells as well as in macrophases. This finding suggests that the cancer cell itself give positive aid to destruction of the stroma.