A study on function mechanism of a novel endogenous

脊髓啡肽是一种新的内源性疼痛调节物质，可调节脑啡肽降解活性。在本研究中，我们发现spinorphin对FMLP刺激的人PMNs的炎症反应有明显的抑制作用，可能是一种新的内源性抗炎肽，部分是通过抑制[Ca^<2+>] i的变化来介导的。接下来，我们研究了spinorphin的作用，使用小鼠气袋作为急性炎症反应模型的体内系统。在这个模型中，卡拉胶和pmn在炎症部位的积累是急性炎症反应的一个指标。卡拉胶诱导的气囊部位静脉注射spinorphin，与盐处理组相比，抑制率为64%。联合使用氨肽酶N抑制剂lehistin可显著抑制卡拉胶诱导的PNMs积累。在未来，spinorphin将成为一个重要的工具，用于澄清神经肽诱导的生物现象，如脑啡肽介导的疼痛和炎症。

Spinorphin is a novel endogenous pain-regulated substance whic modulated an enkephalin-degrading activity. In this study, we found that spinorphin causes a significant inhibition of inflammatory responses by human PMNs stimulated with FMLP and may be a new endogenous anti-inflammatory peptide which is mediated in part through the suppression in [Ca^<2+>] i changes. Next we examined the effects of spinorphin, using an in vivo system involving mouse air-pouch as a model of acute inflammatory response. In this model carrageenan and PMNs accumulation at inflammatory sites was an indicator of an acute inflammatory response. Intravenous administration of spinorphin within air-pouch site elicited by carrageenan, the inhibition being 64%, compared to the saline-treated group. Combination of leuhistin, an inhibitor of aminopeptidase N,significantly suppressed PNMs accumulation induced carrageenan. In the future, spinorphin will be an important tool for use in clarifying of neuropeptide-induced biological phenomena such as enkephalin mediated pain and inflammation.