E.coli Genome Project

大肠杆菌作为一种模式生物，在分子生物学中的一些基本概念的建立中发挥了重要的作用，大量的数据积累有助于更好地了解大肠杆菌和许多其他生物的细胞过程。在这种情况下，完成对大肠杆菌基因组的分析是各种生物基因结构和功能的参考。日本的大肠杆菌基因组计划始于1989年，目标是从0分钟开始顺时针方向分析大肠杆菌基因组序列。1995年3月，测定了遗传图谱上0-13min区的DNA序列。1995年4月，我们成立了一个由12名日本研究人员组成的新小组，使用一种新的方案，在13到69分钟内分析该区域的DNA序列。我们将测序方法分为四个步骤：1)片段准备，2)模板准备，3)测序和4)数据分析和存储。该小组的每个成员负责这些步骤中的一个或两个。13-69分钟区的序列于1997年1月完成，通过将我们的序列数据与布拉特纳小组报道的69-100分钟区的序列数据相结合，完成了连续序列中的大肠杆菌基因组。我们的序列分析发表在四篇独立的论文中，注册在DDBJ上，可以在Web上找到。对整个大肠杆菌基因组的序列分析正在进行中。

Escherichia coli has been playing significant roles as a model organism in establishment of a number of basic concepts in molecular biology, and the huge accumulation of data has contributed to a better understanding of cellular processes of E.coli and numerous other organisms. Such being the case, completion of analysis of the E.coli genome is vital as a reference for structures and functions of genes in various organisms.The E.coli genome project in Japan was begun in 1989, the objective being to analyze the sequence of the E.coli genome from 0 minutes and in a clockwise direction. In March 1995, the DNA sequences of the 0-13 min region on the genetic map had been determined. In April 1995, we set up a new group consisting of 12 Japanese researchers to analyze the DNA sequence of the region from 13 to 69 minutes, using a new protocol. We separated the sequencing protocol into four steps ; 1) Fragment preparation, 2) Template preparation, 3) Sequencing and 4) Analysis and storage of data. Each member of the group is responsible for one or two of these steps.The sequence of the 13-69 min region was completed in January 1997 and the E.coli genome in a contiguous sequence was completed by combining our sequence data with the sequence data of the 69-100 min region reported by Blattner's group. Analysis of our sequences was published in four separate papers, registered in DDBJ and can be found on the Web. Sequence analysis of the entire E.coli genome is under way.