Histological studies on differntiation and systematization of vitamin A-storing cells in vertebrates.

为了了解肝星状细胞中维生素A储存的异质性，我们采用氯化金法对猪肝进行了检测。油浸下进行维生素a脂滴形态测定，计算体积绘制在计算机地图上。肝小叶被细分为5区/5区区室化。值的分布呈现区域梯度，外围1区和2区较高，中间3区向小叶中心4区和5区下降。在单一区域内变化明显，形成区域梯度；门静脉周围比门静脉中部高。沿外周，门静脉入口小静脉量化，门静脉周围的发生率为60%，门静脉中部为5%。在七鳃鳗肾系膜细胞中发现了丰富的维生素A。我们讨论了这种细胞类型与肝星状细胞的相似之处。根据我们目前和以前对七鳃鳗的研究结果，我们得出结论，维生素A储存在各种内脏器官中，而不是在身体器官中。在哺乳动物中，维生素A只储存在肝脏中，然而，在服用过量的维生素A后，内脏器官中的成纤维细胞将其结合并储存在细胞质中。维生素a储存细胞系统是一种只存在于脊椎动物内脏器官的间充质细胞系统，维生素a储存细胞不仅在内脏器官之间存在异质性，而且在单个器官内也存在异质性。

In order to know the heterogeneity of stored vitamin A in the hepatic stellate cells, we examined pig liver by the use of gold chloride method. Vitamin A-lipid droplet morphometry was performed under oil immersion and the calculated volumes were plotted on computerized maps. The hepatic lobule was sub divided into 5-zone/5-region comparmentalization. Distribution of values exhibited a district zonal gradient, higher at peripheral zones 1 and 2, dcreased over intermediate zone 3 toward centrilobular zones 4 and 5. Within a single zone, variations were obvious, forming a regional gradient ; higher at periportal than mideptal regions. Along the periphery, inlet venules of portal veins were quantified, revealing an occurrence rate of 60% at periportal, and 5% at midesptal regions.Abundant vitamin A was found in the mesangial cells in the lamprey kidney. We discussed similarities of the this cell type with the hepatic stellate cell. From our both present and previous findings on the lamprey, we concluded that vitamin A is stored in various splanchnic organs but not in the somatic organs. In the mammalian species, vitamin A is stored only in the liver, however, after the administration of excess vitamin A the fibroblastic cells in the splanchnic organs incorporated and stored it in the cytopalsm. Vitamin A-storing cell system is a system of mesenchymal cell system existing only in the splanchnic organs of vertebrates and vitamin A-storing cells reveal heterogeneity not only among splanchnic organs but also within a single organ.