Development of novel diagnosis method for minutecancer by infrared fluorescence endoscopy using fluorescence-emitting antibody by infrared ray excitation.

本实验研制了ICG-磺酸-OSU(ICG-N-羟基磺基琥珀酰亚胺酯)作为红外线(IR)激发下的荧光标记材料(Bioorg Med Chem Letters 1995；5：2689-94，BioOrganic&Medical Chemical 6(11)：2179-2184,1998)和红外荧光显微镜成像系统(Dig Endosc 1997；278-82)。ICG-Sulo-OSU的荧光强度太低，无法用红外成像系统检测到微小病变。因此，在本研究中，我们开发了一种使用高灵敏度电荷耦合器件(ICCD)(消化内窥镜11,1999(印刷中))和增强剂(生物有机和药物化学6(5)：613-618,1998)的成像系统，可以用于在组织水平上对这种标记物质进行成像。因此，提供了这样一个事实，即一些针对癌细胞的抗体也可以通过使用与ICG结合的这种抗体来通过IR荧光检测癌细胞(J Gasterenterol 1998；33：467-71)。医学调查46,1999(在印)，消化内窥镜检查，11,1999(在印))

ICG-sulfo-OSu (ICG N-hydroxy sulfo succinimide ester), as the labeling material that is fluorescent on excitation with infrared ray(IR) (Bioorg Med Chem Letters 1995 ; 5 : 2689-94, Bioorganic &Medicinal Chemistry 6(11) : 2179-2184, 1998) and the imaging system for IR fluorecent microscope(Dig Endosc 1997 ; 278-82) were developed in this experiment. The intensity of fluorescence of ICG-sulfo-OSu is too low for detection of microlesions by IR imaging system. Therefore, in the present study, we developed an imaging system using a highly sensitive charge-coupled-device (ICCD) (Digestive Endoscopy 11, 1999 (in press)) and the reinforcement agents (Bioorganic &Medicinal Chemistry 6(5) : 613-618, 1998) that can be used to image this labeling substance at the tissue level. Therefore, provide the fact that some antibodies which are specific to cancer cells can detect cancer cells by IR fluorescence also by using such antibodies bound with ICG.(J Gastroenterol 1998 ; 33 : 467-71. J.Medical Investigation 46, 1999 (in press), Digestive Endoscopy 11, 1999 (in press))