Effect of different anesthetics and anesthetic exposure times on survival course of sepsis-analysis of mRNA in mice performed cecal ligation & puncture-

目得：为了确定突变品系小鼠的存活曲线和存活率是否不同，（C3 H/HeJ：内毒素耐药）和内毒素敏感株（C3 H/HeN）（盲肠结扎和穿刺：CLP）是用不同的麻醉剂进行的（氯胺酮或不同的氟烷暴露）。CLP在随机选择的麻醉方法下进行，吸入氟烷氧（15分钟、2小时或6小时）（分别为1.00、120和103只小鼠）或以配对方式在每种品系的8周龄雄性小鼠中单次肌肉注射氯胺酮（127只小鼠）。采用多重比较的方法，比较两种不同麻醉方法CLP后10天内两种菌株的日存活率，以及四种麻醉方法CLP后10天内两种菌株的日存活率。C_3H/HeJ与C_3H/HeN相比，除氟烷2小时麻醉外，其他麻醉方法均延迟死亡（氟烷15分钟和6小时在第1天和第2-5天p<0.01，氯胺酮在第4-6天p<0.05），然而，两种品系中每种麻醉方法的最终存活率相同。无论对内毒素的遗传易感性，短时间暴露于氟烷导致最迅速的死亡相比，其他麻醉方法（P<0.05>，和两个小时的氟烷显示最好的survival.CONCLUSIONS：内毒素的敏感性影响脓毒症的过程中的每一种麻醉方法，和麻醉方法影响的生存从败血症在两种菌株。

PURPOSE : To determine whether the survival curves and rates differ between a mutant strain of mice (C3H/HeJ : endotoxin-resistant) and endotoxin-susceptible strain (C3H/HeN) when severe sepsis (cecal ligation and puncture : CLP) is performed with different anesthetics (ketamine or varied exposure to halothane).METHODS : CLP was performed under a randomly chosen anesthetic method out of inhalation of halothane in oxygen (15 min, 2 hr, or 6 hr) (1.00,120, and 103 mice respectively) or a single injection of ketamine im (127 mice) in paired fashion in 8-week-old male mice of each strain. The daily survival rates were compared between the two strains until the 10th day after CLP for each anesthetic and among the four anesthetic methods for each strain using multiple comparisons.RESULTS : The C3H/HeJ had delayd death relative to the C3H/HeN with every anrsthetic method except a two hour halothane (p<0.01 at day 1 and day 2-5 in halothane 15 min and 6 hr, p<0.05 at day 4-6 in ketamine), however, the final survival rates for each method of anesthesia were the same in the two strains. Regardless of the genetic susceptibility to endotoxin, short exposure to halothane resulted to the most rapid death compared with the other anesthetic methods (p<0.05>, and a two hour halothane showed the best survival.CONCLUSIONS : The endotoxin susceptibility affected the septic course for each anesthetic method, and the anesthetic methods influenced the survival from sepsis in both strains.