Trial study of versatile optical cell and equipment for high pressure spectroscopy

为了将高压光谱学应用于生物化学和食品科学领域的研究，本研究的目的是：1)建立常规使用的高压光谱池，安装在大学、学院实验室中配备的通用光谱分析仪器上。2)高压光谱仪，用于测量从远紫外区到可见光的吸收光谱和荧光光谱。1.建造了带光学窗口的高压容器，以适应从紫外到可见光的吸收光谱仪、荧光光谱仪和圆二向色性偏振仪。使用SUS-630制造容器，其性能如下：最大压力，500兆帕；可测量波长，200-700 nm，内体积。0.5-1.0毫升。专门设计了测量镉的容器。样品和压力介质用自由活塞隔开，不接触。使用不同的窗口材料和尺寸设计了几种容器，并测试了它们的耐压性和易操作性。2.利用光纤系统构建了配备高压容器的高压光谱仪。该仪器仅用于测量生物材料的吸收和荧光。其性能如下：最大压力。用上述两种方法研究了高压对各种蛋白质和酶的影响。但完全满足CD测量的研究必须在未来不断进行。

To apply high pressure spectroscopy to researches in biochemistry and food science fields, this study was intended to construct 1)high pressure optical cells which were routinely used by being set to common spectroscopic apparatuses equipped in laboratories of universities, colleges. and company and government institutes, and 2)a high pressure spectrophotometer dedicated to measure absorption and fluorescence spectroscopy from far ultraviolet region to visible region.1.High pressure vessels with optical windows were constructed to fit an absorption spectrophotometer used in ultraviolet to visible region, a fluorescense spectrometer, and a circular dichroism polarimeter. Vessels were constructed with SUS-630 and their performance was as follows : maximum pressure, 500 MPa ; measurable wavelength, 200-700 nm, inside volume. 0.5-1.0 ml. Vessels for CD measurement was specially designed. Sample and pressure medium were separated by a free piston not to contact them. Several vessels were designed by using various window materials and sizes and their pressure tolerance and handling easiness were tested.2.High pressure spectrometer equipped with a high pressure vessel was constructed by applying an optical fiber system. This is used only in measuring absorption and flurescence of biological materials., The performance was as follows : maximum pressure. 500 MPa and measurable wavelength, 200-700nm.Using two method described above, high pressure effect on various proteins and enzymes were studied. But studies completely satisfying CD measurement had to be continuously in future.