An epidemiological surveillance of lyme borreliosis in wild rodents : especially analysis of the prevalence of the prevalence of harboring organisms and the system of passing the winter

为获得日本野生动物莱姆病的流行病学资料，我们对北方茨木县山区固定地点捕获的野生啮齿动物进行了全年血清学监测和病原体分离。共捕获美姬鼠281只，蒙氏田鼠60只。由于这些啮齿类动物的免疫球蛋白与蛋白G反应，我们尝试用4种疏螺旋体基因型抗原通过PG-ELISA方法进行血清学监测。用改进的PG-ELISA检测了其它野生动物血清，对高山姬鼠和田鼠的抗体阳性率分别为10.3%和21.6%。高山姬鼠高滴度血清阳性率仅为6.9%，而蒙氏田鼠高滴度血清阳性率为46.2%。在月患病率中，OD值的平均值在2月份最低。从5月到7月，OD值的平均值逐渐上升。这些都是一些 ...更多信息 7月至10月的血清样本显示高滴度。10月后，没有血清样本显示如此高的滴度，平均值逐渐下降，直到12月。这一事实支持小型啮齿动物体内莱姆病抗体与蜱类活动、繁殖季节、候鸟等有关，并提示小型啮齿动物体内抗体保存时间不长，体型大小是影响姬鼠体内抗体的重要因素。结果表明，从这些啮齿类动物中均能成功分离到病原体，其中高山姬鼠和蒙氏田鼠的分离率分别为2.1%和5.0%。采用多克隆抗体、PCR、RFLP等方法对分离菌株进行鉴定。结果表明，低温保存对生物体的保存效果无明显影响。表明该菌在蜱体内的保存时间不长，在低温下在动物体内的保存时间较长。因此，这些生物可能在冬季被保存在哺乳动物体内。这些研究是在口头报告和论文，并应在两个文件完成。少

To obtained epidemiological data of Lyme borreliosis about Japanese wild animals, we carried out the serological surveillance and the isolation of organism from wild rodents that were caught at fixed place in mountain area of northern Ibaraki prefecture throughout the year. Two hundred eighty one of Apodemus speciosus and 60 of Microtus montebelli were caught and tested. As the immunoglobulin of these rodents did react with protein G,we attempted a serological surveillance by the PG-ELISA method using 4 borrelia genotype antigens. The other wild animal serum was also tested by this improved PG-ELISA.The antibody positive rates to Apodemus speciosus and Microtus montebelli were 10.3% and 21.6%, respectively. The positive sera indicating high titer were merely 6.9% in Apodemus speciosus, but 46.2% in Microtus montebelli. At the monthly prevalence, the lowest levels of mean of OD values was found in February. The mean of OD values was gradually rising from May until July. These were some … More serum samples indicating high titer from July to October. After October, there was no serum samples indicating so high titer and the mean was gradually going down until December. The fact supports that Lyme borreliosis antibody in small rodents is related with the tick activity, breeding season and migratory bird etc. And it suggests that these rodents have not so long kept the antibodies and that the size was an important facter for the possession of antibody in Apodemus speciosus. The isolation of organisms were successful from these rodents and these isolation ratio at Apodemus speciosus and Microtus montebelli were 2.1% and 5.0%, respectively. The identification of isolates was carried out by the polyclonal antibody assay, PCR and RFLP.The experimental infection was carried out at low temperature. It was found that there was no difference of keeping organisms at low temperature. It indicated that this organisms were not so long keeping in tick, but keeping long term in animals at low temperature. Therefore, the organisms may be kept in the body of mammals in winter. These study was presented at a oral presentation and a paper, and should be done at two paper. Less