Molecular epidemiology of tick borne-emerging infectious disease, Anaplasmosis, Spotted fever, and Lyme borreliosis in East Asian countries.

对东亚地区包括日本在内的水库动物和媒介蜱中人类粒细胞无形体病（HGA）病原、嗜吞噬细胞无形体和回归热病原宫本氏疏螺旋体的流行情况进行了调查。在蒙古国伊尔库茨克、哈巴罗夫斯克和色楞格采集的过溶性硬蜱中检出无原体。在日本高知县，回顾性确认两名患者感染无原体，检测到的病原体与在俄罗斯远东地区收集的蜱虫相似。在伊尔库茨克和哈巴罗夫斯克采集的蜱中检出回归热致伯氏疏螺旋体，提示对蜱叮咬患者的诊断应纳入回归热。在台湾捕获的野生啮齿动物中检测到变异无原体。​斑点热立克次体在碱性条件下提取的多糖抗原可作为斑点elisa血清诊断抗原。

The prevalence of human granulocytic anaplasmosis (HGA) agent, Anaplasma phagocytophilum and relapsing fever agent, Borrelia miyamotoi, in reservoir animals and vector ticks in East Asia, including Japan, were examined. Anaplasma was detected from Ixodes persulcatus ticks collected in Irkutsk, Khabarovsk, and Selenge in Mongolia. In Kochi Prefecture, Japan, two patients were confirmed retrospectively to be infected with Anaplasma and the agent detected was similar to those from ticks collected in far-east Russia. Relapsing fever-causing Borrelia was detected in ticks collected in Irkutsk and Khabarovsk, indicating that relapsing fever should be involved in the diagnosis of tick bite patients. Variant Anaplasma was detected in wild rodents captured in Taiwan. This agent has not been found in ticks collected in Taiwan and also China.Polysaccharide antigen extracted from spotted fever Rickettsia under alkaline condition could be used as antigen for Dot-ELISA-based serodiagnosis.