p16 gene alteration and proliferative potential of gliomas with fluorescence in situ hybridization and immunohistochemical studies.

本研究旨在探讨人类胶质瘤与增殖潜能和p16基因改变的关系。荧光原位杂交（FISH）技术也应用于一些染色体的数值异常。由单克隆抗体MIB-1和MIB-5、抗dna拓扑异构酶II α和抗cdc2抗体测定的胶质瘤的增殖潜能与每种病例的恶性肿瘤的组织学分级密切相关。然而，p16基因产物的免疫阳性与它们的增殖潜能没有明显的相关性。p16基因产物的胞浆内阳性染色，以及核内反应，在恶性肿瘤级别较高的病例中是明显的。结果提示p16基因产物的运输受到干扰。用FISH对石蜡块分离的肿瘤细胞核进行研究，用染色体特异性着丝粒探针观察到在高恶性肿瘤中，7号染色体数量增加，10号染色体数量减少。

This study was performed to investigate correlations with proliferative potential and p16 gene alteration in human gliomas. And fluorescence in situ hybridization (FISH) technique is also applied for numeric abnormality in some chromosomes. Proliferative potential of gliomas determined by the monoclonal antibodies MIB-1 and MIB-5, anti-DNA topoisomerase II alpha and anti-cdc2 antibodies is well correlated with histological grade of malignancy in each cases. Whereas, p16 gene products-immunopositivity is not apparently correlated with their proliferative potential in each cases. Intracytoplasmic positive stainings of p16 gene products, as well as intranuclear reaction, are pronounced in cases with higher grade of malignancy. A distrubance of transportation of the p16 gene products may be suggestive form the results. With FISH study on dissociated tumor nuclei from the paraffin blocks, a gain of number of #7 chromosome and a loss of number of #10 chromosome using chromosome specific centromeric probes are observed in higher grade of malignancy.