Studies on the model for cultured human epidermal allong rafting using hu-PBL-SCID mice

为确定无朗格汉斯细胞培养的人表皮移植物在同种异体受者体内能否长期存活，建立了人外周血淋巴细胞重组的SCID小鼠实验模型。在该模型中，人外周血淋巴细胞在体外用抗CD3单抗激活，然后在体内用抗asialoGM1单抗处理后转移到SCID小鼠体内。外周淋巴器官的流式细胞仪检测显示，移植后2-4周，人外周血淋巴细胞(主要是T细胞)植入。培养的人表皮移植物被未经处理的SCID小鼠接受，当移植到背部肌肉上并用硅移植小室保护时。然而，培养的人表皮移植到异体人PBL构建的SCID小鼠上10天后，组织学证实移植床中有表皮脱落和人CD3+T细胞的渗入。这些结果表明，老鼠。该模型可用于分析培养的人异体表皮移植物的排斥反应机制。

To determine whether Langerhans cell-free cultured human epidermal grafts survive for a prolonged period in allogeneic recipients, an experimental model using SCID mice reconstituted with human peripheral blood lymphocytes (hu-PBL) was established. In this model, hu-PBL were activated with anti-CD3 mAb in vitro and then transferred into SCID mice pretreated with anti-asialoGM1 Ab in vivo. Flow cytometry of the peripheral lymphoid organs demonstrated engraftment of hu-PBL (mainly T cells) 2-4 weeks after the transfer. Cultured human epidermal grafts were accepted by untreated by untreated SCID mice, when transplanted on the dorsal muscle and protected with a silicon transplantation chamber. Ten days after cultured human epidermal grafting on SCID mice reconstituted with allongeneic hu-PBL,however, detachment of the epidermis and infiltrantion of human CD3+ T cells in the graft bed were histologically confirmed. These results suggest that mice. This model will be usefull for analysis of the rejection mechanisms of cultured human epidermal allonrafts.