Estimation of tumor proliferative activity according to expression of cell-cycle-related antigen and selection of fractionation schema in radiation therapy.

我们对肿瘤的增殖和放射治疗进行了各种基础和临床研究，1)为了更好地了解肿瘤细胞在放射治疗后的再生动力学，我们研究了小鼠肿瘤在单剂量照射后细胞增殖参数的变化。作为结论之一，先前静止的细胞被募集到肿瘤的增殖池中，被认为有助于rt后的再生。2)2)用小鼠肿瘤研究了一种抗血管生成药物（TNP-470）与分级RT的联合作用。空气分步放射治疗时给予TPN-470可降低肿瘤控制概率。这一意想不到的结果可能与TNP-470对再氧化的部分抑制有关，因为在缺氧条件下TNP-470对TCD-50没有显著的影响。（参考文献6）3)评估总治疗时间（overall treatment time， OTT）对RT局部控制率的影响，以考虑RT中最优的OTT，分析食管癌、声门癌和子宫癌的急性和晚期毒性。在多因素分析中，OTT是局部控制的重要预后因素。综上所述，OTT应在正常组织和患者的耐受范围内尽可能短。（参考文献4）4)研究了增殖细胞核抗原（PCNA）和Ki-67免疫组化指标与食管癌终期放疗（RT）患者局部控制和生存率的关系。我们收集了65例食管癌患者放疗前的活检资料。PCNA LI与食管癌局部控制的已知预后因素无关。PCNA LI <50%或Ki-67 LI <45%的患者局部复发率明显高于LI较高的患者（p<0.05）。结论：PCNA LI、Ki-67 LI与食管局部控制概率显著相关。经明确RT.PCNA LI治疗的鳞状细胞癌可能是选择食管癌分诊方案的有用参数。少

We have performed various basic and clinical studies on tumor proliferation and radiation therapy,1)To understand better the repopulation kinetics of tumor cells after radiotherapy (RT), we investigated changes in cell proliferative parameters after single-dose irradiation of murine tumors. As one of conclusions, recruitment of previously quiescent cells into the proliferative pool in the tumors was suggested to contribute to repopulation after RT.(ref.2)2)Combined effects of an antiangiogenic agent (TNP-470) with fractionated RT were investigated using a murine tumor. The tumor control probability decreased by administrating TPN-470 during fractionated RT in air. This unexpected result may be contributable to partial inhibition of reoxygenation by TNP-470, because no significant changes in TCD-50 under hypoxic conditions by TNP-470. (ref.6)3)The effects of overall treatment time (OTT) on local control of RT were evaluated to consider optimal OTT in RT.Local control rates and acute and … More late toxicities of esophageal cancer, glottic cancer, and uterine cervical cancer were analyzed. In the multivariate analysis, OTT was a significant prognostic factor for local control. In conclusion, OTT should be kept as short as Possible wiyhin the limits of tolerance of normal tissues and patients. (ref.4)4)The relationship of immunohistochemical indices of proliferating cell nuclear antigen (PCNA) and Ki-67 to local control and survival rates for patients with esophageal squamous cell carcinomas treated by definitive radiotherapy (RT) was investigated. Biopsy materials before RT were obtained from 65 patients with esophageal cancer. The PCNA LI was independent of known prognostic factors on local control for esophageal cancer. Patients with the PCNA LI of<50% or the Ki-67 LI of<45% showed significantly higher local recurrence rates than those with higher LIs (p<0.05). In conclusion, PCNA LI and Ki-67 LI were significantly correlated with local control probabilities in esophageal. squamous cell carcinomas treated by definitive RT.PCNA LI may be a useful parameter for selecting the fractionation scheme of esophageal cancers. Less