The Effect of Hepatocyte Growth Factor on the Development of Lung Injury Induced by Thoracic Irradiation in Mice.

用戊巴比妥麻醉C57 Bl/6 J小鼠，并用剂量在15至30戈伊范围内的胸部照射处理。在接受25和30戈伊治疗的小鼠中，在照射后1至12个月出现体重减轻。30戈伊照射后180天内死亡率为22%~ 40%。外源性人重组肝细胞生长因子（hrHGF，4μg/小鼠/4天，I. v.）在照射后10天用小鼠进行。经30戈伊照射后，所有hrHGF处理的小鼠均存活1年。C3 H/HeN小鼠在15戈伊照射后11天注射和不注射hrHGF（6μg/小鼠/6天）。两组小鼠均存活240天。根据组织学观察，hrHGF注射对急性肺炎和纤维化变化的改善尚不清楚。0戈伊、15戈伊、30戈伊、+HGF 4个剂量组照射后10个月肺组织胶原蛋白相对总蛋白含量无显著差异。HGF可能在照射后早期肺损伤中起保护作用，照射后ICR小鼠肝、肾、肺中内源性HGF浓度与照射剂量和器官类型有关。30戈伊全身照射（TBI）后12小时肝、肾组织中HGF含量暂时升高，48小时肺组织中HGF含量达到最高。2戈伊TBI后72 h仅肺组织HGF表达增加，提示HGF可能是肺组织放射损伤修复的一个因子。

C57Bl/6J mice were anesthetized by nembutal and treated with thoracic irradiation to dose in the range 15 to 30 Gy. In mice treated with 25 and 30 Gy body weight losses were manifest at 1 to 12 months after irradiation. Deaths (22%-40%) were observed in the period 180 days after 30 Gy irradiation. Delayed administration of exogenous human recombinant hepatocyte growth factor (hrHGF, 4μg/mouse/4 days, I.v.) was performed with mice at 10 days after irradiation. All mice treated with hrHGF were lived for 1 year after 30 Gy irradiation. C3H/HeN mice were treated with and without hrHGF injection (6μg/mouse/6 days,) at 11 days after 15 Gy irradiation. Mice both groups were alived for 240 days. Improvement of acute pneumonic and fibrotic changes by hrHGF injection was unclear based on the histological observation. Relative content of collagen to total protein in the lung at 10 months after irradiation was not different among four treatment groups: 0 Gy, 15 Gy, 30 Gy,+HGF. HGF might be give protection of the lung during the early pneumonic phase after irradiation.Concentrations of endogenous HGF in liver, kidney and lung in ICR mice exposed to radiation depended on the dose and the kind of organs. HGF contents in both liver and kidney after 30 Gy total body irradiation (TBI) increased at 12 hrs temporarily, lung reached to maximum at 48 hrs. after 2 Gy TBI, only lung showed the increase of HGF at 72 hrs.HGF may act as a factor for repair of radiation induced injury in the lung.