NON-DESTRUCTIVE METABOLIC ANANLYSIS OF TRANSPLANTED ORGANS USING MULTI-NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY

第一年，我们对保存肝的代谢进行了分析。再灌注后高能代谢产物的恢复率随保存时间的延长而降低。保存肝细胞内pH随乳酸蓄积而变为酸中毒。在Krebs-Henseleit培养液中保存的肝脏，1H-MRS测得的弛豫时间随保存时间延长而延长(保存前274.5(]sy.+)。[)21.3毫秒，24小时保存402.6(对+-)。[)35.2毫秒)。而在UW液中保存的肝脏，保存12小时后略有缩短，12小时后仍保持不变(保存前254.5()SY.+-)。[)21.3毫秒，12小时保存242.6(对+-)。[)31.8ms)。松弛时间的延长与保存肝的体积(g%)有关。松弛时间变化与保存肝细胞内水肿的关系。从这些结果中，我们证实了多核磁共振波谱在无创性观察保存过程中能量代谢、细胞内pH和松弛时间变化方面的有效性。第二年，我们研究了从移植肝脏的体表获取体内MRS的方法。我们用表面线圈的方法明确了通过腹壁获得的磁共振波谱中80%或更多的成分来自大鼠的肝脏。门静脉阻断后，β-ATP峰值下降，30min后几乎稳定在初始水平的20%。我们将这种方法应用于移植的大鼠肝脏。不幸的是，由于大鼠肝移植技术的复杂性，数据并不稳定，但从这两年的结果来看，我们证实了多核磁共振波谱作为移植前和移植后器官无创监测的有效性。

In the first year, we analyzed the metabolism of preserved liver. The recovery of high energy metabolites after reperfusion became lower according to the preservation time. Intracellular pH of the preserving liver changed to accidosis with the accumulation of lactic acid. The relaxation time measured by 1H-MRS of the liver preserved in Krebs-Henseleit Medium prolonged according to the preservation time (pre-preservation 274.5(]SY.+-。[)21.3ms, 24hr preservation 402.6(]SY.+-。[)35.2ms). On the other hand, that of the liver preserved in UW solution slightly shortened until 12hr and it was still constant after 12hr (pre-preservation 254.5(]SY.+-。[)21.3ms, 12hr preservation 242.6(]SY.+-。[)31.8ms). The prolongation of the relaxation time was correlated with the water volume (g%) of the preserved liver. The relationship between the changes in relaxation time and the intracellular edema of the preserved liver. From these results, we confirmed the usefulness of the multi-nuclear magnetic resonance spectroscopy for the non-invasive observation of changes in energy metabolism, intracellular pH and relaxation time during preservation.In the second year, we investigated the methods for obtaining the in vivo MRS of the from body surface which supposed the transplanted liver. We clarified that 80% or more of the components in the obtained MR spectra through the abdominal wall was originated from the rat liver by using surface coil methods. The beta-ATP peak decreased after the portal vein occlusion and it became almost constant at 20% of its initial level after 30 minutes. We applied this method to the transplanted rat livers. Unfortunately the data were not stable because of the complicated technique of the rat liver transplantation.However from the results obtained in these two years, we confirmed the efficacy of the multinuclear magnetic resonance spectroscopy as the non-invasive monitor of transplanted organs before and after transplantation.