Experimental studies to develope a new treatment for the organ transplanataion by the inducing prolonged immuno tolerance and chimerism

尽管免疫抑制剂的研究取得了新的进展，但成功的肠道移植仍需要理想的治疗方法。在不需要延长免疫抑制或广泛的受体预处理的情况下，诱导同种异体移植物的特异性耐受将有望改善肠道移植的效果，减少并发症。本研究的目的是通过建立大鼠活体小肠移植模型，探讨母婴脾移植对活体小肠移植诱导持久的特异性免疫耐受的影响。结果发现，移植的脾在儿童的大网膜中生长良好，而不是成人的，并怀疑儿童的脾起到了排斥母亲移植的作用。我们推测，切除儿童脾和移植一块脾可能会导致活体相关器官移植的长期免疫耐受。需要进一步的实验来评估脾切除和脾移植的适当时机，并阐明母子脾移植在诱导特异性免疫耐受方面的作用。受体脾切除与母子(受体)脾移植相结合，可延长活体小肠移植的免疫耐受时间。

Despite recent developments in immunosuppressive agents, an ideal treatment is still needed for successful intestinal transplantation. The induction of specific tolerance of allograft without the need for prolong immunosupperssion or extensive recipient pretreatment will be expected to improve the result of intestinal transplantation and to reduce the complications. The aim of our study was to clarify the effects of splenotransplantation from mother to child on the induction of prolonged specific immuno-tolerance to living related intestinal transplantation using a rat model.From our results, we found that the transplanted spleen well grew up in child omentum more that adult one's and suspected that the child spleen played to reject the transplanted spleen from mother. We speculated that a combination of extirpation of child spleen and transplantation of a piece of spleen might induce prolonged immunotolerance to living-related organ transplantation. Further experiments are necessary to evaluate the proper timing of splenectomy and splenotransplantation and to clarify the effects of the transplanted spleen from mother to child on inducing specific immunotolerance in a animal model using not only skin transplantation but also organ transplantation as the second transplantation. A combination of recipient splenectomy and splenotransplantaion from mother to child (recipient) might induce prolonged immunotolerance to living-related intestinal transplantation.