Prevention of hypoxic brain damage by in vivo transfection through the carotid artery in cirsulatory arrest

利用日本血凝病毒脂质体经冠状动脉将基因转染到整个心脏的有效方法该方法代表了一种新的心脏体内基因转染技术，因此可能为该项目和研究提供新的工具。体内基因转染热休克蛋白70和bcl-2增强心肌和肝细胞对缺血再灌注损伤的耐受性：大鼠心脏和肝脏中过表达的HSP 70和Bcl-2对缺血再灌注损伤具有保护作用，提示HSP 70和bcl-2基因转染有可能成为一种新的器官保护方法。用脂质体将荧光素标记的寡核苷酸经颈动脉转染到大鼠脑内。热休克蛋白70（HSJ 70）和bcl-2基因在体脑内转染对脑缺血再灌注损伤的保护作用：将HSJ 70和bcl-2基因转染大鼠脑内。

Efficient gene transfer method into the whole heart through the coronary artery with Hemagglutinating Virus of Japan liposoine this method represents a novel in vivo gene transfar technique for the heart and thus may provide a new tool for this project and research.In vivo gene transfection with heat shock protein 70 and bcl-2 enhance myocardial and liver cell tolerance to ischemia-reperfusion injury : overexpressed HSP 70 and Bcl-2 in rat hearts and livers plays a protective role in ischemia- reperfusion injury, suggesting the possibility that gene transfection with HSP 70 and bcl-2 may become a novel method for the organ protection.Gene transfar method into the brain through the carotid artery in hypothermic circulatory arrest HVJ-liposome was used for gene transfection of oligonucleotide labeled with fluorescein into the rat brain through the carotid artery. Fluorescein isothiocyanate was detected in the nuclei.In vivo gene transfection with heat shock protein 70 and bcl-2 into the brain for the protection in ischcmia-reperfusion injury : HSJ 70 and bcl-2 are transfered into the rat brain.